cfpdБюллетень физиологии и патологии дыханияBulletin Physiology and Pathology of Respiration1998-5029Дальневосточный научный центр физиологии и патологии дыхания10.36604/1998-5029-2022-85-37-46cfpd-1038Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHСравнительная характеристика уровней экспрессии TRP каналов на макрофагах больных хронической обструктивной болезнью легкихComparative characteristics of TRP channels expression levels on the macrophages of patients with chronic obstructive pulmonary diseaseНаумовД. Е.NaumovD. E.

Денис Евгеньевич Наумов, канд. мед. наук, зав. лабораторией, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Denis E. Naumov, PhD (Med.), Head of Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

denn1985@bk.ruСугайлоИ. Ю.SugayloI. Yu.

Ивана Юрьевна Сугайло, аспирант, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Ivana Yu. Sugaylo, PhD Student, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

ivanka_888@mail.ruКотоваО. О.KotovaO. O.

Олеся Олеговна Котова, канд. мед. наук, младший научный сотрудник, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Olesya O. Kotova, PhD (Med.), Junior Staff Scientist, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

foxy_voxy_on@mail.ruГассанД. А.GassanD. A.

Дина Анатольевна Гассан, канд. мед. наук, научный сотрудник, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Dina A. Gassan, PhD (Med.), Staff Scientist, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

dani-shi@mail.ruГорчаковаЯ. Г.GorchakovaYa. G.

Яна Геннадьевна Горчакова, лаборант-исследователь, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Yana G. Gorchakova, Research Laboratory Assistant, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

janet.gorchakova@gmail.comМальцеваТ. А.MaltsevaT. A.

Татьяна Анатольевна Мальцева, канд. мед. наук, научный сотрудник, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Tatyana A. Maltseva, PhD (Med.), Staff Scientist, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

malta-82@mail.ruФедеральное государственное бюджетное научное учреждение «Дальневосточный научный центр физиологии и патологии дыхания»РоссияFar Eastern Scientific Center of Physiology and Pathology of RespirationRussian Federation2022220920220853746Copyright © Наумов Д.Е., Сугайло И.Ю., Котова О.О., Гассан Д.А., Горчакова Я.Г., Мальцева Т.А., 20222022Наумов Д.Е., Сугайло И.Ю., Котова О.О., Гассан Д.А., Горчакова Я.Г., Мальцева Т.А.Naumov D.E., Sugaylo I.Y., Kotova O.O., Gassan D.A., Gorchakova Y.G., Maltseva T.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://cfpd.elpub.ru/jour/article/view/1038

Введение. Макрофаги являются одними из ключевых клеток в патогенезе хронической обструктивной болезни легких (ХОБЛ), опосредуя первичный иммунный ответ и координируя дальнейшую реакцию иммунной системы при контакте с сигаретным дымом и аэрополлютантами. Известно, что некоторые каналы TRP, экспрессированные на макрофагах, являются рецепторами пылевых частиц и компонентов сигаретного дыма. Цель. Изучить особенности экспрессии каналов TRPV1, TRPV4, TRPA1 и TRPM8 на макрофагах, дифференцированных из моноцитов периферической крови, и альвеолярных макрофагах больных ХОБЛ и курильщиков, не имеющих бронхиальной обструкции.Материалы и методы. Экспрессию каналов TRP на уровне мРНК изучали в макрофагах, дифференцированных из моноцитов 8 больных ХОБЛ и 6 здоровых курильщиков, методом количественной ПЦР с обратной транскрипцией. Экспрессию каналов TRP на уровне белка исследовали методом непрямой проточной цитометрии на альвеолярных макрофагах 39 больных ХОБЛ и 8 курильщиков без признаков бронхиальной обструкции.Результаты. Установлено, что в условиях in vitro макрофаги, полученные из моноцитов больных ХОБЛ, отличаются достоверным увеличением числа транскриптов TRPV1 в 4,8 раза (p=0,009). При этом экспрессия белка TRPV1 на альвеолярных макрофагах больных ХОБЛ также значимо выше по сравнению с клетками курящих лиц из группы контроля (14,1 [6,4‒21,2]% против 6,1 [2,1‒9,8]%, p=0,006). Кроме этого, мы обнаружили, что экспрессия TRPV4 увеличена среди активных курильщиков, страдающих ХОБЛ, а экспрессия каналов TRPA1 и TRPM8 коррелирует с некоторыми показателями вентиляционной функции легких.Заключение. Полученные результаты свидетельствуют, что повышенная экспрессия TRPV1 на макрофагах может являться маркером заболевания и вносить вклад в его развитие, в то время как экспрессия TRPV4, TRPA1 и TRPM8 может влиять на клиническое течение ХОБЛ.

Introduction. Macrophages are one of the key cells in the pathogenesis of chronic obstructive pulmonary disease (COPD), mediating the primary immune response and coordinating the further reaction of the immune system upon contact with cigarette smoke and air pollutants. It is known that some TRP channels expressed on macrophages are receptors for dust particles and cigarette smoke components.Aim. To study the features of TRPV1, TRPV4, TRPA1 and TRPM8 channels expression on monocyte-derived macrophages and alveolar macrophages of COPD patients and smokers without bronchial obstruction.Materials and methods. Expression of TRP channels at the mRNA level was studied in monocyte-derived macrophages obtained from 8 COPD patients and 6 healthy smokers by quantitative reverse transcription PCR. Expression of TRP channels at the protein level was studied on alveolar macrophages of 39 COPD patients and 8 healthy smokers by indirect flow cytometry.Results. It was found that under in vitro conditions, monocyte-derive macrophages of COPD patients were distinguished by a significant 4.8-fold increase in the number of TRPV1 transcripts (p=0.009). At the same time, the expression of the TRPV1 protein on the alveolar macrophages of COPD patients was also significantly higher when compared to the cells of smokers from the control group (14.1 [6.4‒21.2]% vs. 6.1 [2.1‒9.8]%, p=0.006). In addition, we found that TRPV4 expression was increased among active smokers with COPD, and the expression of TRPA1 and TRPM8 channels correlated with some lung function parameters.Conclusion. The obtained results suggest that the increased expression of TRPV1 on macrophages may be a marker of the disease and contribute to its development, while the expression of TRPV4, TRPA1 and TRPM8 may influence the clinical course of COPD.

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The authors declare that there are no conflicts of interest present.