cfpdБюллетень физиологии и патологии дыханияBulletin Physiology and Pathology of Respiration1998-5029Дальневосточный научный центр физиологии и патологии дыхания10.12737/article_5b96073c5711b1.83866044cfpd-113Research ArticleСтатьиОСОБЕННОСТИ ЭКСПРЕССИИ РЕЦЕПТОРА TRPM8 В РЕСПИРАТОРНОМ ТРАКТЕ БОЛЬНЫХ БРОНХИАЛЬНОЙ АСТМОЙPECULIARITIES OF TRPM8 RECEPTOR EXPRESSION IN THE RESPIRATORY TRACT OF ASTHMA PATIENTSНаумовД. Е.NaumovD. E.denn1985@bk.ruГассанД. А.GassanD. A.noemail@neicon.ruКилимиченкоК. Ф.KilimichenkoK. F.kolemualnaya@mail.ruАфанасьеваЕ. Ю.Afanas'evaE. Yu.noemail@neicon.ruШелудькоЕ. Г.SheludkoE. G.noemail@neicon.ruКолосовВ. П.KolosovV. P.noemail@neicon.ruДальневосточный научный центр физиологии и патологии дыханияFar Eastern Scientific Center of Physiology and Pathology of Respiration2018181120190691924Copyright © Наумов Д.Е., Гассан Д.А., Килимиченко К.Ф., Афанасьева Е.Ю., Шелудько Е.Г., Колосов В.П., 20192019Наумов Д.Е., Гассан Д.А., Килимиченко К.Ф., Афанасьева Е.Ю., Шелудько Е.Г., Колосов В.П.Naumov D.E., Gassan D.A., Kilimichenko K.F., Afanas'eva E.Y., Sheludko E.G., Kolosov V.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cfpd.elpub.ru/jour/article/view/113

Целью настоящего исследования было проанализировать особенности экспрессии рецептора TRPM8 на уровне белка в индуцированной мокроте и назальном эпителии больных бронхиальной астмой (БА), и оценить их взаимосвязь с базисной терапией и холодовой гиперреактивностью дыхательных путей. Было обследовано 43 человека, в том числе лица, больные персистирующей БА легкой и средней тяжести и больные хроническим необструктивным бронхитом (контрольная группа). Анализ экспрессии TRPM8 выполняли методом непрямой проточной цитометрии. Кроме этого, больным проводили спирометрическое исследование и бронхопровокационную пробу с 3-минутной гипервентиляцией холодным воздухом. В результате было установлено, что TRPM8 экспрессирован на макрофагах индуцированной мокроты и назальном эпителии, причем экспрессия среди больных БА, не получавших базисной терапии, выражена в большей степени, по сравнению с больными, получавшими лечение или контрольной группой. Кроме этого, установлена взаимосвязь между повышенным уровнем экспрессии TRPM8 на макрофагах и холодовой гиперреактивностью дыхательных путей. Интерес для дальнейших исследований должно представлять изучение возможности использования TRPM8 как прогностического биомаркера БА на различных этапах развития заболевания.

The aim of the study was to analyze the peculiarities of TRPM8 receptor expression at protein level in induced sputum and nasal epithelium of asthma patients, and to evaluate their relationship to maintenance therapy and cold airway hyperresponsiveness. The study enrolled 43 patients, including those with persistent mild-to-moderate asthma and those with chronic non-obstructive bronchitis (control group). Analysis of TRPM8 expression was performed by indirect flow cytometry. In addition, patients underwent spirometry and a bronchoprovocation test with 3-minute cold air hyperventilation. As a result, we found that TRPM8 was expressed on macrophages of induced spleen and nasal epithelium. Its expression in patients with asthma who did not receive maintenance therapy was more pronounced as compared to the treated patients or the control group. Moreover, the relationship between increased expression of TRPM8 on macrophages and cold airway hyperresponsiveness was established. Further studies should reveal the possibility of TRPM8 usage as a prognostic biomarker of asthma at various stages of the disease development.

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The authors declare that there are no conflicts of interest present.