References

cfpd

Бюллетень физиологии и патологии дыхания

Bulletin Physiology and Pathology of Respiration

1998-5029

Дальневосточный научный центр физиологии и патологии дыхания

cfpd-538

Research Article

Статьи

МЕТОДЫ АНАЛИЗА ПЕРЕКИСНОГО ОКИСЛЕНИЯ ЛИПИДОВ В МЕДИКО-БИОЛОГИЧЕСКИХ ИССЛЕДОВАНИЯХ

METHODS FOR LIPID PEROXIDATION ANALYSIS IN MEDICAL AND BIOLOGICAL RESEARCH

Некрасов

Эдуард Витальевич

Nekrasov

Eduard V.

ed_nekrasov@mail.ru

Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения РАМНFar Eastern Scientific Center of Physiology and Pathology of Respiration SB RAMS

2012

19022020

04698108

2020

Некрасов Э.В.

Nekrasov E.V.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://cfpd.elpub.ru/jour/article/view/538

Представлен краткий обзор методов анализа продуктов свободно-радикального перекисного окисления полиненасыщенных жирных кислот, представляющих интерес для медико-биологических исследований состояний, сопровождающихся окислительным стрессом. Перекисное окисление липидов (ПОЛ) является следствием окислительного стресса и выражается в образовании многочисленных продуктов, различающихся по химической структуре, времени жизни, токсичности и биологической активности. Интенсивность ПОЛ существенно возрастает при многих патологических состояниях. Анализ продуктов ПОЛ представляет интерес для оценки уровня окислительного стресса в организме, для исследования токсического, метаболического и регулирующего действия этого процесса на организм, а также в диагностике некоторых заболеваний. Предложенные методы анализа продуктов ПОЛ различаются по специфичности, чувствительности, сложности исполнения и необходимого оборудования. В массовых клинических исследованиях предпочтение отдается менее трудоемким и специфичным методам анализа стабильных продуктов ПОЛ, хотя возрастающее применение физико-химических методов анализа, прежде всего масс-спектрометрии, делает возможным одновременный анализ широкого круга продуктов ПОЛ. Введение в практику высокоспецифичных антител делает иммуноферментный анализ многих крайне минорных и сложно определяемых продуктов ПОЛ рутинным занятием. Достоверность и точность анализа во многом определяются предпринятыми при отборе и хранении проб мерами, предотвращающими распад или дальнейшее образование продуктов ПОЛ в образце.

A brief review of methods for analysis of products of free-radical polyunsaturated fatty acids peroxidation which are of some interest for medical and biological studies of the states which are accompanied by oxidative stress is presented. Lipid peroxidation (LPO) is the consequence of oxidative stress and results in the formation of numerous products which differ in structure, life time, toxicity and biological activity. LPO significantly accelerates at different diseases. Analysis of LPO products is of interest for the estimation of oxidative stress, for the research of toxic, metabolic and signaling effects of the products on an organism, and for diagnosis of different diseases. Multiple methods for analysis of LPO products have been developed. They vary in specificity, sensitivity, complexity of procedure and equipment. In clinical studies, less complicated and specific methods for analysis of stable LPO products are preferred, though increasing application of more advanced techniques like mass spectrometry gives opportunity for the analysis of wide spectrum of LPO products. Development of highly specific antibodies makes ELISA a common tool of analysis of minor and otherwise difficult-to-determine LPO products. Reliability and precision of analysis depend highly on precautions taken on sample handling, preparation and storage, which are to avoid degradation or further formation of LPO products.

перекисное окисление липидовгидроперекисиизопростаныальдегидыметоды анализаlipid peroxidationhydroperoxidesisoprostanesaldehydesanalytical methods

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The authors declare that there are no conflicts of interest present.