References

cfpd

Бюллетень физиологии и патологии дыхания

Bulletin Physiology and Pathology of Respiration

1998-5029

Дальневосточный научный центр физиологии и патологии дыхания

cfpd-551

Research Article

Статьи

ТРАНСПОРТ ЛИПИДОВ В ФЕТОПЛАЦЕНТАРНОМ БАРЬЕРЕ С ПОМОЩЬЮ ЛИПИДПЕРЕНОСЯЩИХ БЕЛКОВ H-FABP У БЕРЕМЕННЫХ С ОБОСТРЕНИЕМ ЦИТОМЕГАЛОВИРУСНОЙ ИНФЕКЦИИ В ТРЕТЬЕМ ТРИМЕСТРЕ ГЕСТАЦИИ

LIPIDS TRANSPORTATION WITHIN FETOPLACENTAL BARRIER WITH THE HELP OF H-FABP LIPID-TRANSFER PROTEINS IN PREGNANT WOMEN WITH THE EXACERBATION OF CYTOMEGALOVIRUS INFECTION IN THE THIRD TRIMESTER OF PREGNANCY

Луценко

Михаил Тимофеевич

Lutsenko

Mikhail T.

Lucenkomt@mail.ru

Довжикова

Инна Викторовна

Dovzhikova

Inna V.

dncfpd@ramn.ru

Андриевская

Ирина Анатольевна

Andrievskaya

Irina A.

dncfpd@ramn.ru

Ишутина

Наталия Александровна

Ishutina

Nataliya A.

ishutina-na@mail.ru

Бабенко

Ольга Петровна

Babenko

Ol'ga P.

olga2274.74@yandex.ru

Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения РАМНFar Eastern Scientific Center of Physiology and Pathology of Respiration SB RAMS

2013

19022020

0476271

2020

Луценко М.Т., Довжикова И.В., Андриевская И.А., Ишутина Н.А., Бабенко О.П.

Lutsenko M.T., Dovzhikova I.V., Andrievskaya I.A., Ishutina N.A., Babenko O.P.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://cfpd.elpub.ru/jour/article/view/551

В работе проанализированы теоретические концепции о транспорте жирных кислот, а также представлены собственные данные. Изучен характер переноса жирных кислот с помощью липидпереносящих белков (H-FABP) в синцитиотрофобласте ворсинок плаценты при обострении цитомегаловирусной инфекции в третьем триместре беременности. Обследовано 25 беременных в третьем триместре гестации с обострением цитомегаловирусной инфекции при росте титра антител класса G 1:800 (основная группа), и 20 беременных, не болевших на протяжении всего периода гестации (контрольная группа). Титр антител класса М и G к цитомегаловирусу и индекс авидности определяли иммуноферментным методом, измерение содержания липидпереносящего белка проводили на спектрофотометре. Гистохимическая реакция на определение активности перекисного окисления в синцитиотрофобласте и эндотелии кровеносных сосудов пуповины выполнялась методом Винклера-Шульце. Количественное содержание перекисей проводилось методом компьютерной цитофотометрии. Установлено, что при обострении цитомегаловирусной инфекции у беременных в третьем триместре гестации снижается поступление в синцитиотрофобласт ворсинок плаценты липидпереносящих белков. Отмечается избирательная передача жирных кислот через внутреннюю мембрану синцитиотрофобласта в пуповинную кровь плода. При обострении у беременных цитомегаловирусной инфекции в крови плода снижается количество противовоспалительных жирных кислот семейства ω-3 и, напротив, увеличивается поступление провоспалительных жирных кислот семейства ω-6 и арахидоновой кислоты.

The work deals with theoretical conceptions about fat acids transportation as well as personal investigation data. The character of fat acids transportation was studied with the help of lipid- transfer proteins H-FABR in the syncytiotrophoblast of placenta villi at the exacerbation of cytomegalovirus infection in the third trimester of pregnancy. 25 pregnant women in the third trimester of gestation with the exacerbation of cytomegalovirus infection at the growth of antibodies titer of G 1:800 class (the main group) and 20 women who were not sick for the whole period of gestation (the control group) were examined. Antibodies titer of M and G class to cytomegalovirus and avidity index were found by immune-enzyme method; the measurement of lipid-transfer protein content was done at spectrophotometer. The histochemical reaction to find out the activity of peroxidation in the syncytiotrophoblast and umbilical cord blood vessels endothelium was done by Winkler-Schulz method. The quantitative estimation of peroxides was conducted with the computer cytophotometry method. It was found out that at the exacerbation of cytomegalovirus infection in pregnant women in the third trimester of gestation there is a decrease of lipid-transfer proteins into syncytiotrophoblast of placenta villi. There is also a selective transportation of fat acids through inner membrane of syncytiotrophoblast into the fetus umbilical blood. At the exacerbation of cytomegalovirus infection in pregnant women there is a drop of anti-inflammatory fat acids of ω-3 family and vice versa there is an increase of anti-inflammatory acids of ω-6 family and arachidonic acid.

цитомегаловирусная инфекцияплаценталипидпереносящие белкижирные кислотыcytomegalovirus infectionplacentafatty acidlipid-transfer proteins

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The authors declare that there are no conflicts of interest present.