cfpdБюллетень физиологии и патологии дыханияBulletin Physiology and Pathology of Respiration1998-5029Дальневосточный научный центр физиологии и патологии дыхания10.36604/1998-5029-2020-78-31-39cfpd-832Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHОсобенности экспрессии каналов TRPV1, TRPV4, TRPM8 и TRPA1 в макрофагах, полученных из моноцитов больных хронической обструктивной болезнью легкихPeculiarities of TRPV1, TRPV4, TRPM8 and TRPA1 expression in monocyte-derived macrophages from COPD patientsСугайлоИ. Ю.SugayloI. Yu.

Ивана Юрьевна Сугайло, лаборант-исследователь, лаборатория молекулярных и трансляционных исследований  

675000, г. Благовещенск, ул. Калинина, 22

Ivana Yu. Sugaylo, Assistant Researcher, Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

ivanka_888@mail.ruКотоваО. О.KotovaO. O.

Олеся Олеговна Котова, младший научный сотрудник, лаборатория молекулярных и трансляционных исследований  

675000, г. Благовещенск, ул. Калинина, 22

Olesya O. Kotova, MD, Junior Staff Scientist, Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

foxy_voxy_on@mail.ruГассанД. А.GassanD. A.

Дина Анатольевна Гассан, канд. мед. наук., научный сотрудник, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Dina A. Gassan, MD, PhD (Med.), Staff Scientist, Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

dani-shi@mail.ruНаумовД. Е.NaumovD. E.

Денис Евгеньевич Наумов, канд. мед. наук, зав. лабораторией, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Denis E. Naumov, MD, PhD (Med.), Head of Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

denn1985@bk.ruАфанасьеваЕ. Ю.Afanas’evaE. Yu.

Евгения Юрьевна Афанасьева, младший научный сотрудник, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Evgeniya Yu. Afanas’eva, MD, Junior Staff Scientist, Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

evgeniyananev@yandex.ruМальцеваТ. А.MaltsevaT. A.

Татьяна Анатольевна Мальцева, канд. мед. наук, научный сотрудник, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Tatyana A. Maltseva, MD, PhD (Med.), Staff Scientist, Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

malta-82@mail.ruФедеральное государственное бюджетное научное учреждение «Дальневосточный научный центр физиологии и патологии дыхания»Far Eastern Scientific Center of Physiology and Pathology of Respiration2020171220200783139Copyright © Сугайло И.Ю., Котова О.О., Гассан Д.А., Наумов Д.Е., Афанасьева Е.Ю., Мальцева Т.А., 20202020Сугайло И.Ю., Котова О.О., Гассан Д.А., Наумов Д.Е., Афанасьева Е.Ю., Мальцева Т.А.Sugaylo I.Y., Kotova O.O., Gassan D.A., Naumov D.E., Afanas’eva E.Y., Maltseva T.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cfpd.elpub.ru/jour/article/view/832моноцитымакрофагиTRP каналывоспалениесигаретный дымХОБЛmonocytesmacrophagesTRP channelsinflammationcigarette smokeCOPDReferencesMills C.D., Kincaid K., Alt J.M., Heilman M.J., Hill A.M. M-1/M-2 macrophages and the Th1/Th2 paradigm // J. Immunol. 2000. Vol.164, №12. P.6166–6173. doi: 10.4049/jimmunol.164.12.6166Mills C.D., Kincaid K., Alt J.M., Heilman M.J., Hill A.M. M-1/M-2 macrophages and the Th1/Th2 paradigm. J. Immunol. 2000; 164(12):6166–6173. doi: 10.4049/jimmunol.164.12.6166Gao J., Scheenstra M.R., van Dijk A., Veldhuizen E.J.A., Haagsman H.P. A new and efficient culture method for porcine bone marrow-derived M1- and M2-polarized macrophages // Vet. Immunol. Immunopathol. 2018. Vol.200. P.7–15. doi: 10.1016/j.vetimm.2018.04.002Gao J., Scheenstra M.R., van Dijk A., Veldhuizen E.J.A., Haagsman H.P. A new and efficient culture method for porcine bone marrow-derived M1- and M2-polarized macrophages. Vet. Immunol. Immunopathol. 2018; 200:7–15. doi: 10.1016/j.vetimm.2018.04.002Poltavets A.S., Vishnyakova P.A., Elchaninov A.V., Sukhikh G.T., Fatkhudinov T.K. Macrophage Modification Strategies for Efficient Cell Therapy // Cells. 2020. Vol.9, №6. Article number 1535. doi: 10.3390/cells9061535Poltavets A.S., Vishnyakova P.A., Elchaninov A.V., Sukhikh G.T., Fatkhudinov T.K. Macrophage Modification Strategies for Efficient Cell Therapy. Cells 2020; 9(6):1535. doi: 10.3390/cells9061535Rőszer T. Understanding the Mysterious M2 Macrophage through Activation Markers and Effector Mechanisms // Mediators Inflamm. 2015. Vol.2015. Article number 816460. doi: 10.1155/2015/816460Rőszer T. Understanding the Mysterious M2 Macrophage through Activation Markers and Effector Mechanisms. Mediators Inflamm. 2015; 2015:816460. doi: 10.1155/2015/816460Xue J., Schmidt S.V., Sander J., Draffehn A., Krebs W., Quester I., De Nardo D., Gohel T.D., Emde M., Schmidleithner L., Ganesan H., Nino-Castro A., Mallmann M.R., Labzin L., Theis H., Kraut M., Beyer M., Latz E., Freeman T.C., Ulas T., Schultze J.L. Transcriptome-based network analysis reveals a spectrum model of human macrophage activation // Immunity. 2014. Vol.40, №2. P.274–288. doi: 10.1016/j.immuni.2014.01.006Xue J., Schmidt S.V., Sander J., Draffehn A., Krebs W., Quester I., De Nardo D., Gohel T.D., Emde M., Schmidleithner L., Ganesan H., Nino-Castro A., Mallmann M.R., Labzin L., Theis H., Kraut M., Beyer M., Latz E., Freeman T.C., Ulas T., Schultze J.L. Transcriptome-based network analysis reveals a spectrum model of human macrophage activation. Immunity 2014; 40(2):274–288. doi: 10.1016/j.immuni.2014.01.006Yamasaki K., Eeden S.F.V. Lung Macrophage Phenotypes and Functional Responses: Role in the Pathogenesis of COPD // Int. J. Mol. Sci. 2018. Vol.19, №2. Article number 582. doi: 10.3390/ijms19020582Yamasaki K., Eeden S.F.V. Lung Macrophage Phenotypes and Functional Responses: Role in the Pathogenesis of COPD. Int. J. Mol. Sci. 2018; 19(2):582. doi: 10.3390/ijms19020582Yoshida T., Tuder R.M. Pathobiology of cigarette smoke-induced chronic obstructive pulmonary disease // Physiol. Rev. 2007. Vol.87, №3. P.1047–1082. doi: 10.1152/physrev.00048.2006Yoshida T., Tuder R.M. Pathobiology of cigarette smoke-induced chronic obstructive pulmonary disease. Physiol. Rev. 2007; 87(3):1047–1082. doi: 10.1152/physrev.00048.2006Richens T.R., Linderman D.J., Horstmann S.A., Lambert C., Xiao Y.Q., Keith R.L., Boé D.M., Morimoto K., Bowler R.P., Day B.J., Janssen W.J., Henson P.M., Vandivier R.W. Cigarette smoke impairs clearance of apoptotic cells through oxidant-dependent activation of RhoA // Am. J. Respir. Crit. Care Med. 2009. Vol.179, №11. P.1011–1021. doi: 10.1164/rccm.200807-1148OCRichens T.R., Linderman D.J., Horstmann S.A., Lambert C., Xiao Y.Q., Keith R.L., Boé D.M., Morimoto K., Bowler R.P., Day B.J., Janssen W.J., Henson P.M., Vandivier R.W. Cigarette smoke impairs clearance of apoptotic cells through oxidant-dependent activation of RhoA. Am. J. Respir. Crit. Care Med. 2009; 179(11):1011–1021. doi: 10.1164/rccm.200807-1148OCСугайло И.Ю., Наумов Д.Е. Современные представления о роли каналов с транзиторным рецепторным потенциалом в патогенезе хронической обструктивной болезни легких (обзор литературы) // Бюллетень физиологии и патологии дыхания. 2019. Вып.74. С.119–130. https://doi.org/10.36604/1998-5029-2019-74-119-130Sugaylo I.Yu., Naumov D.E. Modern concepts of the role of transient receptor potential channels in chronic obstructive pulmonary disease pathogenesis (review). Bûlleten' fiziologii i patologii dyhaniâ=Bulletin Physiology and Pathology of Respiration 2019; (74):119–130 (in Russian). https://doi.org/10.36604/1998-5029-2019-74-119-130Li M., Li Q., Yang G., Kolosov V.P., Perelman J.M., Zhou X.D. Cold temperature induces mucin hypersecretion from normal human bronchial epithelial cells in vitro through a transient receptor potential melastatin 8 (TRPM8)-mediated mechanism // J. Allergy Clin. Immunol. 2011. Vol.128, №3. P.626–634.e1-5. doi: 10.1016/j.jaci.2011.04.032Li M., Li Q., Yang G., Kolosov V.P., Perelman J.M., Zhou X.D. Cold temperature induces mucin hypersecretion from normal human bronchial epithelial cells in vitro through a transient receptor potential melastatin 8 (TRPM8)-mediated mechanism. J. Allergy Clin. Immunol. 2011; 128(3):626–634.e1-5. doi: 10.1016/j.jaci.2011.04.032Zhu G.; ICGN Investigators, Gulsvik A., Bakke P., Ghatta S., Anderson W., Lomas D.A., Silverman E.K., Pillai S.G. Association of TRPV4 gene polymorphisms with chronic obstructive pulmonary disease // Hum. Mol. Genet. 2009. Vol.18, №11. P.2053–2062. doi: 10.1093/hmg/ddp111Zhu G.; ICGN Investigators, Gulsvik A., Bakke P., Ghatta S., Anderson W., Lomas D.A., Silverman E.K., Pillai S.G. Association of TRPV4 gene polymorphisms with chronic obstructive pulmonary disease. Hum. Mol. Genet. 2009; 18(11): 2053–2062. doi: 10.1093/hmg/ddp111Xiong M., Guo M., Huang D., Li J., Zhou Y. TRPV1 genetic polymorphisms and risk of COPD or COPD combined with PH in the Han Chinese population // Cell Cycle. 2020. Vol.19, №22. P.3066–3073. doi: 10.1080/15384101.2020.1831246Xiong M., Guo M., Huang D., Li J., Zhou Y. TRPV1 genetic polymorphisms and risk of COPD or COPD combined with PH in the Han Chinese population. Cell Cycle 2020; 19(22):3066–3073. doi: 10.1080/15384101.2020.1831246Naumov D., Kotova O., Gassan D., Sheludko E., Afanaseva E., Maltseva T., Sugaylo I. Role of TRPM8 polymorphisms in predisposition to COPD development in smokers // Eur. Respir. J. 2020. Vol.56, Suppl.64. Article number 1128. doi: 10.1183/13993003.congress-2020.1128Naumov D., Kotova O., Gassan D., Sheludko E., Afanaseva E., Maltseva T., Sugaylo I. Role of TRPM8 polymorphisms in predisposition to COPD development in smokers. Eur. Respir. J. 2020; 56(Suppl.64):1128. doi: 10.1183/13993003.congress-2020.1128Naumov D., Gassan D., Kotova O., Afanaseva E., Sheludko E., Sugaylo I., Perelman J. Effect of TRPA1 and TRPM8 polymorphisms on lung function in COPD // Eur. Respir. J. 2020. Vol.56, Suppl.64. Article number 1129. doi: 10.1183/13993003.congress-2020.1129Naumov D., Gassan D., Kotova O., Afanaseva E., Sheludko E., Sugaylo I., Perelman J. Effect of TRPA1 and TRPM8 polymorphisms on lung function in COPD. Eur. Respir. J. 2020; 56(Suppl.64): 1129. doi: 10.1183/13993003.congress-2020.1129da Silva C.O., Gicquel T., Daniel Y., Bártholo T., Vène E., Loyer P., Pôrto L.C., Lagente V., Victoni T. Alteration of immunophenotype of human macrophages and monocytes after exposure to cigarette smoke // Sci. Rep. 2020. Vol.10, №1. Article number 12796. doi: 10.1038/s41598-020-68753-1da Silva C.O., Gicquel T., Daniel Y., Bártholo T., Vène E., Loyer P., Pôrto L.C., Lagente V., Victoni T. Alteration of immunophenotype of human macrophages and monocytes after exposure to cigarette smoke. Sci. Rep. 2020; 10(1):12796. doi: 10.1038/s41598-020-68753-1Day A., Barnes P., Donnely L. COPD monocytes differentiate into pro-inflammatory macrophages regardless of environment // Eur. Respir. J. 2013. Vol.42, Suppl.57. Article number 3873.Day A., Barnes P., Donnely L. COPD monocytes differentiate into pro-inflammatory macrophages regardless of environment. Eur. Respir. J. 2013; 42(Suppl.57):3873.Soodaeva S., Postnikova L., Boldina M., Kubysheva N., Li T., Kilimanov I., Nikitina L. Serum IL-17 and IL-18 levels in asthma-COPD overlap syndrome patients // Eur. Respir. J. 2015. Vol.46, Suppl.59. PA4886. doi: 10.1183/13993003.congress-2015.PA4886Soodaeva S., Postnikova L., Boldina M., Kubysheva N., Li T., Kilimanov I., Nikitina L. Serum IL-17 and IL-18 levels in asthma-COPD overlap syndrome patients. Eur. Respir. J. 2015; 46(Suppl.59): PA4886. doi: 10.1183/13993003.congress-2015.PA4886Yanagisawa H., Hashimoto M., Minagawa S., Takasaka N., Ma R., Moermans C., Ito S., Araya J., Budelsky A., Goodsell A., Baron J.L., Nishimura S.L. Role of IL-17A in murine models of COPD airway disease // Am. J. Physiol. Lung Cell. Mol. Physiol. 2017. Vol.312, №1. P.L122–L130. doi: 10.1152/ajplung.00301.2016Yanagisawa H., Hashimoto M., Minagawa S., Takasaka N., Ma R., Moermans C., Ito S., Araya J., Budelsky A., Goodsell A., Baron J.L., Nishimura S.L. Role of IL-17A in murine models of COPD airway disease. Am. J. Physiol. Lung Cell. Mol. Physiol. 2017; 312(1):L122–L130. doi: 10.1152/ajplung.00301.2016Christenson S.A., van den Berge M., Faiz A., Inkamp K., Bhakta N., Bonser L.R., Zlock L.T., Barjaktarevic I.Z., Barr R.G., Bleecker E.R., Boucher R.C., Bowler R.P., Comellas A.P., Curtis J.L., Han M.K., Hansel N.N., Hiemstra P.S., Kaner R.J., Krishnanm J.A., Martinez F.J., O'Neal W.K., Paine R. 3rd, Timens W., Wells J.M., Spira A., Erle D.J., Woodruff P.G. An airway epithelial IL-17A response signature identifies a steroid-unresponsive COPD patient subgroup // J. Clin. Invest. 2019. Vol.129, №1. P.169–181. doi: 10.1172/JCI121087Christenson S.A., van den Berge M., Faiz A., Inkamp K., Bhakta N., Bonser L.R., Zlock L.T., Barjaktarevic I.Z., Barr R.G., Bleecker E.R., Boucher R.C., Bowler R.P., Comellas A.P., Curtis J.L., Han M.K., Hansel N.N., Hiemstra P.S., Kaner R.J., Krishnanm J.A., Martinez F.J., O'Neal W.K., Paine R. 3rd, Timens W., Wells J.M., Spira A., Erle D.J., Woodruff P.G. An airway epithelial IL-17A response signature identifies a steroid-unresponsive COPD patient subgroup. J. Clin. Invest. 2019; 129(1):169–181. doi: 10.1172/JCI121087Wei B., Sheng Li C. Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease // J. Int. Med. Res. 2018. Vol.46, №9. P.3890–3902. doi: 10.1177/0300060518781642Wei B., Sheng Li C. Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease. J. Int. Med. Res. 2018; 46(9):3890–3902. doi: 10.1177/0300060518781642Boonen B., Alpizar Y.A., Sanchez A., López-Requena A., Voets T., Talavera K. Differential effects of lipopolysaccharide on mouse sensory TRP channels // Cell Calcium. 2018. Vol.73. P.72–81. doi: 10.1016/j.ceca.2018.04.004Boonen B., Alpizar Y.A., Sanchez A., López-Requena A., Voets T., Talavera K. Differential effects of lipopolysaccharide on mouse sensory TRP channels. Cell Calcium 2018; 73:72–81. doi: 10.1016/j.ceca.2018.04.004Nassini R., Pedretti P., Moretto N., Fusi C., Carnini C., Facchinetti F., Viscomi A.R., Pisano A.R., Stokesberry S., Brunmark C., Svitacheva N., McGarvey L., Patacchini R., Damholt A.B., Geppetti P., Materazzi S. Transient receptor potential ankyrin 1 channel localized to non-neuronal airway cells promotes non-neurogenic inflammation // PLoS One. 2012. Vol.7, №8. e42454. doi: 10.1371/journal.pone.0042454Nassini R., Pedretti P., Moretto N., Fusi C., Carnini C., Facchinetti F., Viscomi A.R., Pisano A.R., Stokesberry S., Brunmark C., Svitacheva N., McGarvey L., Patacchini R., Damholt A.B., Geppetti P., Materazzi S. Transient receptor potential ankyrin 1 channel localized to non-neuronal airway cells promotes non-neurogenic inflammation. PLoS One 2012; 7(8):e42454. doi: 10.1371/journal.pone.0042454Wang Q., Chen K., Zhang F., Peng K., Wang Z., Yang D., Yang Y. TRPA1 regulates macrophages phenotype plasticity and atherosclerosis progression // Atherosclerosis. 2020. Vol.301. P.44–53. doi: 10.1016/j.atherosclerosis.2020.04.004Wang Q., Chen K., Zhang F., Peng K., Wang Z., Yang D., Yang Y. TRPA1 regulates macrophages phenotype plasticity and atherosclerosis progression. Atherosclerosis 2020; 301:44–53. doi: 10.1016/j.atherosclerosis.2020.04.004Ninomiya Y., Tanuma S.I., Tsukimoto M. Differences in the effects of four TRPV1 channel antagonists on lipopolysaccharide-induced cytokine production and COX-2 expression in murine macrophages // Biochem. Biophys. Res. Commun. 2017. Vol.484, №3. P.668–674. doi: 10.1016/j.bbrc.2017.01.173Ninomiya Y., Tanuma S.I., Tsukimoto M. Differences in the effects of four TRPV1 channel antagonists on lipopolysaccharide-induced cytokine production and COX-2 expression in murine macrophages. Biochem. Biophys. Res. Commun. 2017; 484(3):668–674. doi: 10.1016/j.bbrc.2017.01.173Fernandes E.S., Liang L., Smillie S.J., Kaiser F., Purcell R., Rivett D.W., Alam S., Howat S., Collins H., Thompson S.J., Keeble J.E., Riffo-Vasquez Y., Bruce K.D., Brain S.D. TRPV1 deletion enhances local inflammation and accelerates the onset of systemic inflammatory response syndrome // J. Immunol. 2012. Vol.188, №11. P.5741–5751. doi: 10.4049/jimmunol.1102147Fernandes E.S., Liang L., Smillie S.J., Kaiser F., Purcell R., Rivett D.W., Alam S., Howat S., Collins H., Thompson S.J., Keeble J.E., Riffo-Vasquez Y., Bruce K.D., Brain S.D. TRPV1 deletion enhances local inflammation and accelerates the onset of systemic inflammatory response syndrome. J. Immunol. 2012; 188(11):5741–5751. doi: 10.4049/jimmunol.1102147Hamanaka K., Jian M.Y., Townsley M.I., King J.A., Liedtke W., Weber D.S., Eyal F.G., Clapp M.M., Parker J.C. TRPV4 channels augment macrophage activation and ventilator-induced lung injury // Am. J. Physiol. Lung Cell. Mol. Physiol. 2010. Vol.299, №3. P.L353–362. doi: 10.1152/ajplung.00315.2009Hamanaka K., Jian M.Y., Townsley M.I., King J.A., Liedtke W., Weber D.S., Eyal F.G., Clapp M.M., Parker J.C. TRPV4 channels augment macrophage activation and ventilator-induced lung injury. Am. J. Physiol. Lung Cell. Mol. Physiol. 2010; 299(3): L353–362. doi: 10.1152/ajplung.00315.2009Scheraga R.G., Abraham S., Niese K.A., Southern B.D., Grove L.M., Hite R.D., McDonald C., Hamilton T.A., Olman M.A. TRPV4 Mechanosensitive Ion Channel Regulates Lipopolysaccharide-Stimulated Macrophage Phagocytosis // J. Immunol. 2016. Vol.196, №1. P.428–436. doi: 10.4049/jimmunol.1501688Scheraga R.G., Abraham S., Niese K.A., Southern B.D., Grove L.M., Hite R.D., McDonald C., Hamilton T.A., Olman M.A. TRPV4 Mechanosensitive Ion Channel Regulates Lipopolysaccharide-Stimulated Macrophage Phagocytosis. J. Immunol. 2016; 196(1):428–436. doi: 10.4049/jimmunol.1501688

The authors declare that there are no conflicts of interest present.