cfpdБюллетень физиологии и патологии дыханияBulletin Physiology and Pathology of Respiration1998-5029Дальневосточный научный центр физиологии и патологии дыхания10.36604/1998-5029-2020-78-40-46cfpd-833Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHАнализ уровней экспрессии генов TRPV в респираторном эпителии больных бронхиальной астмой с осмотической гиперреактивностью дыхательных путейAnalysis of TRPV gene expression in the respiratory epithelium of asthma patients with osmotic airway hyperresponsivenessКотоваО. О.KotovaO. O.

Олеся Олеговна Котова, младший научный сотрудник, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Olesya O. Kotova, MD, Junior Staff Scientist, Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

foxy_voxy_on@mail.ruНаумовД. Е.NaumovD. E.

Денис Евгеньевич Наумов, канд. мед. наук, зав. лабораторией, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Denis E. Naumov, MD, PhD (Med.), Head of Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

denn1985@bk.ruАфанасьеваЕ. Ю.Afanas’evaE. Yu.

Евгения Юрьевна Афанасьева, младший научный сотрудник, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Evgeniya Yu. Afanas’eva, MD, Junior Staff Scientist, Laboratory of Molecular and Translational Research 

22 Kalinina Str., Blagoveshchensk, 675000

evgeniyananev@yandex.ruОдиреевА. Н.OdireevA. N.

Андрей Николаевич Одиреев, д-р мед. наук, зав. лабораторией, лаборатория профилактики неспецифических заболеваний легких 

675000, г. Благовещенск, ул. Калинина, 22

Andrey N. Odireev, MD, PhD, D.Sc. (Med.), Head of Laboratory of Prophylaxis of Non-Specific Lung Diseases 

22 Kalinina Str., Blagoveshchensk, 675000

bulleten@mail.ruПерельманЮ. М.PerelmanJ. M.

Юлий Михайлович Перельман, член-корреспондент РАН, д-р мед. наук, профессор, зам. директора по научной работе, зав. лабораторией, лаборатория функциональных методов исследования дыхательной системы

675000, г. Благовещенск, ул. Калинина, 22

Juliy M. Perelman, MD, PhD, D.Sc. (Med.), Corresponding member of RAS, Рrofessor, Deputy Director on Scientific Work, Head of Laboratory of Functional Research of Respiratory System 

22 Kalinina Str., Blagoveshchensk, 675000

jperelman@mail.ruФедеральное государственное бюджетное научное учреждение «Дальневосточный научный центр физиологии и патологии дыхания»Far Eastern Scientific Center of Physiology and Pathology of Respiration2020171220200784046Copyright © Котова О.О., Наумов Д.Е., Афанасьева Е.Ю., Одиреев А.Н., Перельман Ю.М., 20202020Котова О.О., Наумов Д.Е., Афанасьева Е.Ю., Одиреев А.Н., Перельман Ю.М.Kotova O.O., Naumov D.E., Afanas’eva E.Y., Odireev A.N., Perelman J.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cfpd.elpub.ru/jour/article/view/833бронхиальная астмагиперреактивность дыхательных путейосморецепцияTRPV1TRPV2TRPV4экспрессияэпителийasthmaairway hyperresponsivenessosmoreceptionTRPV1TRPV2TRPV4expressionepitheliumReferencesGlobal Initiative for Asthma (GINA). Global Strategy for Asthma Management and Prevention (Update 2020). URL: www.ginasthma.org.Global Initiative for Asthma (GINA). Global Strategy for Asthma Management and Prevention (Update 2020). Available at: www.ginasthma.org.Хижняк Ю.Ю., Перельман Ю.М., Колосов В.П. Сезонная динамика проходимости и реактивности дыхательных путей у больных бронхиальной астмой в условиях муссонного климата // Тихоокеанский медицинский журнал. 2009. №1(35). С.82–84.Khizhniak J., Perelman J., Kolosov V. The seasonal dynamics of airway patency and reactivity in patients with bronchial asthma under monsoon climate conditions. Pacific Medical Journal 2009; (1):82–84 (in Russian).Lam H.C., Li A.M., Chan E.Y., Goggins W.B. 3rd. The short-term association between asthma hospitalisations, ambient temperature, other meteorological factors and air pollutants in Hong Kong: a time-series study // Thorax. 2016. Vol.71, №12. P.1097–1109. doi: 10.1136/thoraxjnl-2015-208054Lam H.C., Li A.M., Chan E.Y., Goggins W.B. 3rd. The short-term association between asthma hospitalisations, ambient temperature, other meteorological factors and air pollutants in Hong Kong: a time-series study. Thorax 2016; 71(12):1097–1109. doi:10.1136/thoraxjnl-2015-208054Bodaghkhani E., Mahdavian M., MacLellan C., Farell A., Asghari S. Effects of meteorological factors on hospitalizations in adult patients with asthma: a systematic review // Can. Respir J. 2019. Vol.2019. Article ID 3435103. doi: 10.1155/2019/3435103Bodaghkhani E., Mahdavian M., MacLellan C., Farell A., Asghari S. Effects of Meteorological Factors on Hospitalizations in Adult Patients with Asthma: A Systematic Review. Can. Respir. J. 2019; 2019:3435103. doi:10.1155/2019/3435103Zhang Y., Peng L., Kan H., Xu J., Chen R., Liu Y., Wang W. Effects of meteorological factors on daily hospital admissions for asthma in adults: a time-series analysis // PLoS One. 2014. Vol.9, №7. P.e102475. doi: 10.1371/journal.pone.0102475Zhang Y., Peng L., Kan H., Xu J., Chen R., Liu Y., Wang W. Effects of meteorological factors on daily hospital admissions for asthma in adults: a time-series analysis. PLoS One 2014; 9(7):e102475. doi:10.1371/journal.pone.0102475Brannan J.D., Lougheed M.D. Airway hyperresponsiveness in asthma: mechanisms, clinical significance, and treatment // Front. Physiol. 2012. Vol.3. P.460. doi: 10.3389/fphys.2012.00460Brannan J.D., Lougheed M.D. Airway hyperresponsiveness in asthma: mechanisms, clinical significance, and treatment. Front. Physiol. 2012; 3:460. doi:10.3389/fphys.2012.00460Anderson S.D. Indirect challenge tests: airway hyperresponsiveness in asthma: its measurement and clinical significance // Chest. 2010. Vol.138, Suppl.2. P.25S–30S. doi: 10.1378/chest.10-0116Anderson S.D. Indirect challenge tests: Airway hyperresponsiveness in asthma: its measurement and clinical significance. Chest 2010; 138(2 Suppl):25S–30S. doi:10.1378/chest.10-0116Liedtke W., Choe Y., Martí-Renom M.A., Bell A.M., Denis C.S., Sali A., Hudspeth A.J., Friedman J.M., Heller S. Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor // Cell. 2000. Vol.103, №3. P.525–535. doi:10.1016/s0092-8674(00)00143-4Liedtke W., Choe Y., Martí-Renom M.A., Bell A.M., Denis C.S., Sali A., Hudspeth A.J., Friedman J.M., Heller S. Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor. Cell 2000; 103(3):525–535. doi:10.1016/s0092-8674(00)00143-4Muraki K., Iwata Y., Katanosaka Y., Ito T., Ohya S., Shigekawa M., Imaizumi Y. TRPV2 is a component of osmotically sensitive cation channels in murine aortic myocytes // Circ. Res. 2003. Vol.93. P.829–838. doi:10.1161/01.RES.0000097263.10220.0CMuraki K., Iwata Y., Katanosaka Y., Ito T., Ohya S., Shigekawa M., Imaizumi Y. TRPV2 is a component of osmotically sensitive cation channels in murine aortic myocytes. Circ. Res. 2003; 93(9):829–838. doi:10.1161/01.RES.0000097263.10220.0CNishihara E., Hiyama T.Y., Noda M. Osmosensitivity of transient receptor potential vanilloid 1 is synergistically enhanced by distinct activating stimuli such as temperature and protons // PLoS One. 2011. Vol.6, №7. P.e22246. doi:10.1371/journal.pone.0022246Nishihara E., Hiyama T.Y., Noda M. Osmosensitivity of transient receptor potential vanilloid 1 is synergistically enhanced by distinct activating stimuli such as temperature and protons. PLoS One 2011; 6(7):e22246. doi:10.1371/journal.pone.0022246McGarvey L.P., Butler C.A., Stokesberry S., Polley L., McQuaid S., Abdullah H., Ashraf S., McGahon M.K., Curtis T.M., Arron J., Choy D., Warke T.J., Bradding P., Ennis M., Zholos A., Costello R.W., Heaney L.G. Increased expression of bronchial epithelial transient receptor potential vanilloid 1 channels in patients with severe asthma // J. Allergy Clin. Immunol. 2014. Vol.133, №3. P.704–712.e4. doi:10.1016/j.jaci.2013.09.016McGarvey L.P., Butler C.A., Stokesberry S., Polley L., McQuaid S., Abdullah H., Ashraf S., McGahon M.K., Curtis T.M., Arron J., Choy D., Warke T.J., Bradding P., Ennis M., Zholos A., Costello R.W., Heaney L.G. Increased expression of bronchial epithelial transient receptor potential vanilloid 1 channels in patients with severe asthma. J. Allergy Clin. Immunol. 2014; 133(3):704–712.e4. doi:10.1016/j.jaci.2013.09.016.Alenmyr L., Uller L., Greiff L., Högestätt E.D., Zygmunt P.M. TRPV4-mediated calcium influx and ciliary activity in human native airway epithelial cells // Basic Clin. Pharmacol. Toxicol. 2014. Vol.114, №2. P.210–216. doi:10.1111/bcpt.12135Alenmyr L., Uller L., Greiff L., Högestätt E.D., Zygmunt P.M. TRPV4-mediated calcium influx and ciliary activity in human native airway epithelial cells. Basic Clin. Pharmacol. Toxicol. 2014; 114(2):210–216. doi:10.1111/bcpt.12135Groneberg D.A., Niimi A., Dinh Q.T., Cosio B., Hew M., Fischer A., Chung K.F. Increased expression of transient receptor potential vanilloid-1 in airway nerves of chronic cough // Am. J. Respir. Crit. Care Med. 2004. Vol.170, №12. P.1276–1280. doi:10.1164/rccm.200402-174OCGroneberg D.A., Niimi A., Dinh Q.T., Cosio B., Hew M., Fischer A., Chung K.F. Increased expression of transient receptor potential vanilloid-1 in airway nerves of chronic cough. Am. J. Respir. Crit. Care Med. 2004; 170(12):1276–1280. doi:10.1164/rccm.200402-174OCJia Y., Wang X., Varty L., Rizzo C.A., Yang R., Correll C.C., Phelps P.T., Egan R.W., Hey J.A. Functional TRPV4 channels are expressed in human airway smooth muscle cells // Am. J. Physiol. Lung Cell. Mol. Physiol. 2004. Vol.287, №2. P.L272–L278. doi:10.1152/ajplung.00393.2003Jia Y., Wang X., Varty L., Rizzo C.A., Yang R., Correll C.C., Phelps P.T., Egan R.W., Hey J.A. Functional TRPV4 channels are expressed in human airway smooth muscle cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 2004; 287(2):L272–L278. doi:10.1152/ajplung.00393.2003Cai X., Yang Y.C., Wang J.F., Wang Q., Gao J., Fu W.L., Zhu Z.Y., Wang Y.Y., Zou M.J., Wang J.X., Xu D.Q., Xu D.G. Transient receptor potential vanilloid 2 (TRPV2), a potential novel biomarker in childhood asthma // J. Asthma. 2013. Vol.50, №2. P.209–214. doi:10.3109/02770903.2012.753454Cai X., Yang Y.C., Wang J.F., Wang Q., Gao J., Fu W.L., Zhu Z.Y., Wang Y.Y., Zou M.J., Wang J.X., Xu D.Q., Xu D.G. Transient receptor potential vanilloid 2 (TRPV2), a potential novel biomarker in childhood asthma. J. Asthma 2013; 50(2):209–214. doi:10.3109/02770903.2012.753454Link T.M., Park U., Vonakis B.M., Raben D.M., Soloski M.J., Caterina M.J. TRPV2 has a pivotal role in macrophage particle binding and phagocytosis // Nat. Immunol. 2010. Vol.11, №3. P.232–239. doi:10.1038/ni.1842Link T.M., Park U., Vonakis B.M., Raben D.M., Soloski M.J., Caterina M.J. TRPV2 has a pivotal role in macrophage particle binding and phagocytosis. Nat. Immunol. 2010; 11(3):232–239. doi:10.1038/ni.1842Xie F., Xiao P., Chen D., Xu L., Zhang B. miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs // Plant Mol. Biol. 2012. Vol.80. P.75–84. doi:10.1007/s11103-012-9885-2Xie F., Xiao P., Chen D., Xu L., Zhang B. miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Mol. Biol. 2012; 80:75–84. doi:10.1007/s11103-012-9885-2Belvisi M.G., Birrell M.A., Wortley M.A., Maher S.A., Satia I., Badri H., Holt K., Round P., McGarvey L., Ford J., Smith J.A. XEN-D0501, a Novel Transient Receptor Potential Vanilloid 1 Antagonist, Does Not Reduce Cough in Patients with Refractory Cough // Am. J. Respir. Crit. Care Med. 2017. Vol.196, №10. P.1255–1263. doi:10.1164/rccm.201704-0769OCBelvisi M.G., Birrell M.A., Wortley M.A., Maher S.A., Satia I., Badri H., Holt K., Round P., McGarvey L., Ford J., Smith J.A. XEN-D0501, a Novel Transient Receptor Potential Vanilloid 1 Antagonist, Does Not Reduce Cough in Patients with Refractory Cough. Am. J. Respir. Crit. Care Med. 2017; 196(10):1255–1263. doi:10.1164/rccm.201704-0769OCZhang L., Sun T., Liu L., Wang L. The research of the possible mechanism and the treatment for capsaicin-induced cough // Pulm. Pharmacol. Ther. 2018. Vol.49. P.1–9. doi:10.1016/j.pupt.2017.12.008Zhang L., Sun T., Liu L., Wang L. The research of the possible mechanism and the treatment for capsaicin-induced cough. Pulm. Pharmacol. Ther. 2018; 49:1–9. doi:10.1016/j.pupt.2017.12.008

The authors declare that there are no conflicts of interest present.