cfpdБюллетень физиологии и патологии дыханияBulletin Physiology and Pathology of Respiration1998-5029Дальневосточный научный центр физиологии и патологии дыхания10.36604/1998-5029-2020-78-128-134cfpd-845Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHСравнительная характеристика условий выделения моноцитов методом адгезии для экспериментов in vitroComparative characteristics of monocytes isolation conditions by adhesion method for in vitro experimentsГассанД. А.GassanD. A.

Дина Анатольевна Гассан, канд. мед. наук, научный сотрудник, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Dina A. Gassan, MD, PhD (Med.), Staff Scientist, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

dani-shi@mail.ruКотоваО. О.KotovaO. O.

Олеся Олеговна Котова, младший научный сотрудник, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Olesya O. Kotova, MD, Junior Staff Scientist, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

foxy_voxy_on@mail.ruНаумовД. Е.NaumovD. E.

Денис Евгеньевич Наумов, канд. мед. наук, зав. лабораторией, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Denis E. Naumov, MD, PhD (Med.), Head of Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

denn1985@bk.ruСугайлоИ. Ю.SugayloI. Yu.

Ивана Юрьевна Сугайло, лаборант-исследователь, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Ivana Yu. Sugaylo, Assistant Researcher, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

ivanka_888@mail.ruГорчаковаЯ. Г.GorchakovaY. G.

Яна Геннадьевна Горчакова, лаборант-исследователь, лаборатория молекулярных и трансляционных исследований

675000, г. Благовещенск, ул. Калинина, 22

Yana G. Gorchakova, Assistant Researcher, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

yana.janet.gorchakova@gmail.comСинюкА. А.SinyukA. A.

Анастасия Андреевна Синюк, младший научный сотрудник, лаборатория молекулярных и трансляционных исследований 

675000, г. Благовещенск, ул. Калинина, 22

Anastasia A. Sinyuk, MD, Junior Staff Scientist, Laboratory of Molecular and Translational Research

22 Kalinina Str., Blagoveshchensk, 675000

amur.asua@gmail.comФедеральное государственное бюджетное научное учреждение «Дальневосточный научный центр физиологии и патологии дыхания»Far Eastern Scientific Center of Physiology and Pathology of Respiration202021122020078128134Copyright © Гассан Д.А., Котова О.О., Наумов Д.Е., Сугайло И.Ю., Горчакова Я.Г., Синюк А.А., 20202020Гассан Д.А., Котова О.О., Наумов Д.Е., Сугайло И.Ю., Горчакова Я.Г., Синюк А.А.Gassan D.A., Kotova O.O., Naumov D.E., Sugaylo I.Y., Gorchakova Y.G., Sinyuk A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cfpd.elpub.ru/jour/article/view/845моноцитымакрофагиадгезиявоспалениеmonocytesmacrophagesadhesioninflammationReferencesMurray P.J., Wynn T.A. Protective and pathogenic functions of macrophage subsets // Nat. Rev. Immunol. 2011. Vol.11, №11. P.723–737. doi: 10.1038/nri3073Murray P.J., Wynn T.A. Protective and pathogenic functions of macrophage subsets. Nat. Rev. Immunol. 2011; 11(11):723–737. doi: 10.1038/nri3073Bikash T., Keunwook L. Metabolic influence on macrophage polarization and pathogenesis // BMB Rep. 2019. Vol.52, №6. P.360–372. doi: 10.5483/BMBRep.2019.52.6.140Bikash T., Keunwook L. Metabolic influence on macrophage polarization and pathogenesis. BMB Rep. 2019; 52(6):360–372. doi: 10.5483/BMBRep.2019.52.6.140Tarique A.A., Logan J., Thomas E., Holt P.G., Sly P.D., Fantino E. Phenotypic, functional, and plasticity features of classical and alternatively activated human macrophages // Am. J. Respir. Cell Mol. Biol. 2015. Vol.53, №5. P.676–88. doi: 10.1165/rcmb.2015-0012OCTarique A.A., Logan J., Thomas E., Holt P.G., Sly P.D., Fantino E. Phenotypic, functional, and plasticity features of classical and alternatively activated human macrophages. Am. J. Respir. Cell Mol. Biol. 2015; 53(5):676–88. doi: 10.1165/rcmb.2015-0012OCBöyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g // Scand. J. Clin. Lab. Invest. Suppl. 1968. Vol.97. P.77–89.Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand. J. Clin. Lab. Invest. Suppl. 1968; 97:77–89.Grievink H.W., Luisman T., Kluft C., Moerland M., Malone K.E. Comparison of Three Isolation Techniques for Human Peripheral Blood Mononuclear Cells: Cell Recovery and Viability, Population Composition, and Cell Functionality // Biopreserv. Biobank. 2016. Vol.14, №5. P.410–415. doi: 10.1089/bio.2015.0104Grievink H.W., Luisman T., Kluft C., Moerland M., Malone K.E. Comparison of Three Isolation Techniques for Human Peripheral Blood Mononuclear Cells: Cell Recovery and Viability, Population Composition, and Cell Functionality. Biopreserv. Biobank. 2016; 14(5):410–415. doi: 10.1089/bio.2015.0104Nielsen M.C., Andersen M.N., Møller H.J. Monocyte isolation techniques significantly impact the phenotype of both isolated monocytes and derived macrophages in vitro // Immunology. 2020. Vol.159, №1. P.63–74. doi: 10.1111/imm.13125Nielsen M.C., Andersen M.N., Møller H.J. Monocyte isolation techniques significantly impact the phenotype of both isolated monocytes and derived macrophages in vitro. Immunology 2020; 159(1):63–74. doi: 10.1111/imm.13125Meital L.T., Coward A.S., Windsor M.T., Bailey T.G., Kuballa A., Russell F.D. A simple and effective method for the isolation and culture of human monocytes from small volumes of peripheral blood // J. Immunol. Methods. 2019. Vol.472. P.75–78. doi: 10.1016/j.jim.2019.04.005Meital L.T., Coward A.S., Windsor M.T., Bailey T.G., Kuballa A., Russell F.D. A simple and effective method for the isolation and culture of human monocytes from small volumes of peripheral blood. J. Immunol. Methods 2019; 472:75–78. doi: 10.1016/j.jim.2019.04.005Treves A.J., Yagoda D., Haimovitz A., Ramu N., Rachmilewitz D., Fuks Z. The isolation and purification of human peripheral blood monocytes in cell suspension // J. Immunol. Methods. 1980. Vol.39, №1-2. P.71–80. doi: 10.1016/0022-1759(80)90295-1Treves A.J., Yagoda D., Haimovitz A., Ramu N., Rachmilewitz D., Fuks Z. The isolation and purification of human peripheral blood monocytes in cell suspension. J. Immunol. Methods 1980; 39(1-2):71–80. doi: 10.1016/0022-1759(80)90295-1Saghaeian-Jazi M., Mohammadi S., Sedight S. Culture and Differentiation of Monocyte Derived Macrophages Using Human Serum: An Optimized Method // Zahedan J. Res. Med. Sci. 2016. Vol.18, №6. P.e7362. doi: 10.17795/zjrms-7362.Saghaeian-Jazi M., Mohammadi S., Sedight S. Culture and Differentiation of Monocyte Derived Macrophages Using Human Serum: An Optimized Method. Zahedan J. Res. Med. Sci. 2016; 18(6):e7362. doi: 10.17795/zjrms-7362Schmidt D., Joyce E.J., Kao W.J. Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro // Acta Biomater. 2011. Vol.7, №2. P.515–525. doi: 10.1016/j.actbio.2010.08.022Schmidt D., Joyce E.J., Kao W.J. Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro. Acta Biomater. 2011; 7(2):515–525. doi: 10.1016/j.actbio.2010.08.022Look N. Heat inactivation – are you wasting your time? // Art to Science (HyClone Newsletter). 1996. Vol.15. P.1–5.Look N. Heat inactivation – are you wasting your time? Art to Science (HyClone Newsletter) 1996; 15:1–5.Koyama Y., Norose-Toyoda K., Hirano S., Kobayashi M., Ebihara T., Someki I., Fujisaki H., Irie S. Type I collagen is a non-adhesive extracellular matrix for macrophages // Arch. Histol. Cytol. 2000. Vol.63, №1. P.71–79. doi: 10.1679/aohc.63.71Koyama Y., Norose-Toyoda K., Hirano S., Kobayashi M., Ebihara T., Someki I., Fujisaki H., Irie S. Type I collagen is a non-adhesive extracellular matrix for macrophages. Arch. Histol. Cytol. 2000; 63(1):71–79. doi: 10.1679/aohc.63.71Garnotel R., Rittié L., Poitevin S., Monboisse J.C., Nguyen P., Potron G., Maquart F.X., Randoux A., Gillery P. Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95) // J. Immunol. 2000. Vol.164, №11. P.5928–5934. doi: 10.4049/jimmunol.164.11.5928Garnotel R., Rittié L., Poitevin S., Monboisse J.C., Nguyen P., Potron G., Maquart F.X., Randoux A., Gillery P. Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J. Immunol. 2000; 164(11):5928–5934. doi: 10.4049/jimmunol.164.11.5928Khalaji S., Zondler L., KleinJan F., Nolte U., Mulaw M.A., Danzer K.M., Weishaupt J.H., Gottschalk K.E. Age Increases Monocyte Adhesion on Collagen // Sci. Rep. 2017. Vol.7. P.46532. doi: 10.1038/srep46532Khalaji S., Zondler L., KleinJan F., Nolte U., Mulaw M.A., Danzer K.M., Weishaupt J.H., Gottschalk K.E. Age Increases Monocyte Adhesion on Collagen. Sci. Rep. 2017; 7:46532. doi: 10.1038/srep46532

The authors declare that there are no conflicts of interest present.