Influence of single nucleotide polymorphisms of innate immunity and inflammatory response genes on humoral and cellular immune parameters in umbilical cord blood of newborns
https://doi.org/10.36604/1998-5029-2026-100-121-128
Abstract
Introduction. The development of the immune system of the fetus and newborn is a complex, multifactorial process in which heredity plays a key role.
Aim. To study the influence of single nucleotide polymorphisms (SNPs) in genes of innate immunity and inflammatory response on humoral and cellular parameters of umbilical cord blood of newborns.
Materials and methods. The study sample consisted of venous umbilical cord blood from 80 infants born at 38-40 weeks of gestation. Total immunoglobulin (Ig) G, M, and A concentrations in plasma were determined using enzyme-linked immunosorbent assay. Lymphocyte subpopulation composition was assessed using flow cytometry. SNP analysis of target genes was determined using polymerase chain reaction (PCR) with high-resolution melting (HRM) analysis or linear-after-the-exponential PCR (LATE-PCR) with fluorescent probes.
Results. Carriage of the G allele of IL6 rs2069837 SNP is associated with a lower concentration of total IgG (β = -1.81, p = 0.03). The minor G allele of TLR4 rs4986790 is associated with an increased level of total IgM in cord blood (β = 0.36, p = 0.04). The C allele of IL10 rs3024498 SNP is associated with an increase in the proportion of total T-lymphocytes (p = 0.006) and T-helpers (p = 0.04) along with a decreased content of NK cells (p = 0.02) in the umbilical cord blood of newborns.
Conclusions. The obtained data indicate the possible contribution of some SNPs of the IL6, TLR4 and IL10 genes to changes in humoral and cellular parameters of the umbilical cord blood of newborns, which justifies the need for further research aimed at confirming the identified associations and developing prognostic models for assessing the immune status of children at risk.
Supporting agencies: The study was carried out under the State Assignment (No. 124101100335-3)
About the Authors
O. O. Nekrasova
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Olesya O. Nekrasova, PhD (Med.), Senior Staff Scientist, Laboratory of Mechanisms of Virus-Associated Developmental Pathologies
22 Kalinina Str., Blagoveshchensk, 675000
D. A. Gassan
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Dina A. Gassan, PhD (Med.), Head of Laboratory, Laboratory of Mechanisms of Virus-Associated Developmental Pathologies
22 Kalinina Str., Blagoveshchensk, 675000
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Review
For citations:
Nekrasova O.O., Gassan D.A. Influence of single nucleotide polymorphisms of innate immunity and inflammatory response genes on humoral and cellular immune parameters in umbilical cord blood of newborns. Bulletin Physiology and Pathology of Respiration. 2026;(100):121-128. (In Russ.) https://doi.org/10.36604/1998-5029-2026-100-121-128
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