ACTIVITY OF HEXOKINASE IN SYNCYTIOTROPHOBLAST OF PLACENTA AND ERYTHROCYTES OF THE PERIPHERAL BLOOD OF PREGNANT WOMEN IN THE THIRD TRIMESTER WITH ACTIVE CYTOMEGALOVIRUS INFECTION

The article shows the data which reflect the state of the carbohydrate metabolism initiated by hexokinase of glucose phosphorylation and the causes of its dysfunction in placenta and erythrocytes of the peripheral blood of women in the third trimester of gestation with active cytomegalovirus infection. After the histochemical study of 75 placentas of women who suffered the recurrence of cytomegalovirus infection in the third trimester of gestation (the main group), a reliable decrease of reaction intensiveness to hexokinase in comparison with the control group of 30 seronegative pregnant women (79.8±1.9 and 209.8±12.1 standard units, р<0.001) was revealed. One of the main causes which leads to the breakage of energetic production can be antigen-mediate hypersecretion of cortisol by adrenal cortex and its output in big quantities into peripheral blood (1467.47.4±8.23 nmole/l in the main group and 730.41±9.04 nmole/l in the control group, p<0.001), which provoked the development of lipid peroxidation processes and the formation of free-radical products contributing to the modification of membrane and cytosolic protein components in placenta. In the peripheral blood of pregnant women from the main group there was an intensification of lipid peroxidation processes which cause the growth of malonic dialdehyde till 1.65±0.09 mcmole/ml (in the control group it was till 0.74±0.05 mcmole/ml, p<0.001) and inactivating antioxidant properties of superoxide dismutase (the decrease till 243.85±7.71 Units/gНb, in the control group it was till 386.77±13.12 Units/gНb, p<0.001) and oleic acid (the drop till 15.22±0.15%, in the control group it was till 20.51±0.21%, p<0.01). At the same time in the main group there was the decrease of ATP pool in erythrocytes till 0.46±0.015 mcmole/ml (in the control group it was till 0.85±1.0 mcmole/ml, р<0.001), which determined the drop of discocytes circulation and the increase of a number of transformed and degenerative cellular forms. The obtained results prove the role of cytomegalovirus infection in the development of oxidative stress which disturbs the regulation of hexokinase ATP-D-glucose-transferring enzyme process, which results in a significant drop of ATP in placenta and erythrocytes of the peripheral blood of pregnant women.

цитомегаловирусная инфекция, гексокиназа, плацента, эритроциты, cytomegalovirus infection, hexokinase, placenta, erythrocytes

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