TRAHEAL EPITHELIUM STEREOLOGICAL PARAMETERS IN BODY COOLING

Morphological, morphometric and stereological characteristics of ciliated epithelial cells of the tracheal mucosa in albino rats during cooling of the body was studied. Three groups of laboratory animals were cooled for 3 hours a day at -15°C during 7, 14 and 21 days. After the experiment the stains from paraffin sections were made. With light microscopy planimetric and stereological quantities in the epithelial layer from the experimental and intact animals were measured. The measured values in the experimental groups were compared with the intact group. It was found out that at 7-day cooling epithelial height decreased by 33%; the size and roundness of ciliated cells increased against intact group by 48% and 49%, respectively. The volume density of epithelium decreased by 24%, the surface density by 12%, but the length density increased by 60%. At 14-day cooling the height was reduced by 51%, the area of ciliated cells decreased by 59%, and the roundness decreased by 25%. The volume density of epithelium decreased by 22%, the surface density by 24%; and the length density increased by 26%. Three-week cooling caused the reduction in the height of the epithelium by 34%.The area of ciliated cells was less than the control by 39%. The roundness exceeded the control by only 4%. The volume density of the epithelium decreased by 34%, the surface density by 37%; and length density increased by 13%. Among measured stereological parameters only the surface density was the most objective parameter of adapting epithelium as it has had the linear depending on the longitude of cold exposure.

эпителий слизистой оболочки трахеи, охлаждение организма, адаптация дыхательной системы, морфометрия, стереология, tracheal mucosa epithelium, cooling of the body, airway adaptation, morphometry, stereology

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