HORMONAL FUNCTION OF PLACENTA AT THE EXACERBATION OF CYTOMEGALOVIRUS INFECTION IN THE GESTATION PERIOD
Abstract
The aim of the work is to analyze the influence of chronic cytomegalovirus infection during exacerbation on synthesis and transformation of steroid hormones, particularly progesterone, in placenta. The villous chorion was the material for the research. It was taken from the medical abortions of 30 pregnant women at the gestation of 11-12 weeks with chronic cytomegalovirus infection at exacerbation (the main group) and from 32 pregnant women at the same period of gestation without the pathology (the control group). The verification of cytomegalovirus, the identification of type-specific antibodies of M and G class and avidity index were carried out by immune-enzyme analysis; cytomegalovirus DNA was identified by PCR. The study of progesterone and cholesterol was done by spectrophotometric method. The analysis of cholesterol synthesis was conducted by histochemical method with the tetrazolium salts on cryostat sections. The activity of 3β-hydroxysteroid dehydrogenase and dehydrogenase of 5β-pregnen-3,20-dione was defined by histochemical method of Z.Lojda et al. with the use of 2 mM solution of Δ5pregnen-3β-ol-20-one and 5β-pregnen-3,20-dione. The exacerbation of cytomegalovirus infection in the first trimester of gestation led to the decrease of the cholesterol synthesis activity. As a result the decrease of the quantity of this predecessor of steroid hormones in the peripheral blood and placenta (р<0.05) was found out. Alongside the decrease of intensiveness of pregnelone transformation into progesterone was found. The decrease of hormone concentration in placenta was also identified (р<0.05). At the same time there was the lowering of the activity of 5β-pregn-3,20-dione transformation, which led to the disturbance of progesterone binding with receptors. Thus, at the exacerbation of cytomegalovirus infection there was a sharp drop of progesterone synthesis, which could create the threat to embryo death at the early stages of gestation.
About the Authors
Mikhail T. Lutsenko
Far Eastern Scientific Center of Physiology and Pathology of Respiration SB RAMS
Russian Federation
Russian Federation
Inna V. Dovzhikova
Far Eastern Scientific Center of Physiology and Pathology of Respiration SB RAMS
Russian Federation
Russian Federation
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Review
For citations:
Lutsenko M.T., Dovzhikova I.V. HORMONAL FUNCTION OF PLACENTA AT THE EXACERBATION OF CYTOMEGALOVIRUS INFECTION IN THE GESTATION PERIOD. Bulletin Physiology and Pathology of Respiration. 2014;(52):62-67. (In Russ.)
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