THE ESTIMATION OF CARBONIC ANHYDRASE AND ITS INFLUENCE ON THE PROCCESSES OF OXYGENATION OF BLOOD ERYTHROCYTES OF PREGNANT WOMEN AT CYTOMEGALOVIRUS INFECTION

Abstract

The aim of the research is to develop histochemical method of estimation of carbonic anhydrase in blood erythrocytes to predict the development of hemic hypoxia in pregnant women under the relapse of cytomegalovirus infection (CMVI) at the 8-9^th weeks. 40 women were examined, including 25 women with CMV-seropositive pregnant women with the relapse of CMVI at the 8-9^th weeks and 15 seronegative pregnant women at the same gestation stage. CMV verification, the identification of type-specific antibodies of IgM and IgG, of avidity index was done by the method of immune-enzyme analysis, of CMV DNA by PCR method, of carbonic anhydrase in blood erythrocytes by Kurate method (in the modification of M.T.Lutsenko, I.A.Andriyevskaya), of oxyhemoglobin concentration by the method of Malloy-Evelyn. As a result there was found the decrease of the number of blood erythrocytes in pregnant women under the relapse of CMVI at the 8-9^th weeks till 65.0±2.2% (in the control it was 85.0±3.2%, p<0.001), who had a response to carbonic anhydrase at the level of 0.015±0.002 arb. units (in the control it was 0.059±0.004 arb. units, p<0.001), and the decrease of oxyhemoglobin concentration till 93.0±1.5% (in the control it was 99.0±1.0%, p<0.001). Thus, the development of hemic hypoxia of pregnant women under the relapse of CMVI at the 8-9^th weeks is conditioned by the suppression of carbonic anhydrase activity in erythrocytes, which leads to the accumulation of aminoformic complexes, the decrease of hemoglobin oxygen affinity and the formation of oxyhemoglobin.