MORPHOFUNCTIONAL CHARACTERISTIC OF SPLEEN IN THE CONDITIONS OF HYPERCHOLESTEREMIA

Abstract

Morphofunctional state of lymphatic corpuscles of the spleen in the conditions of experimental hypercholesteremia was studied. 20 rabbits of Chinchilla breed participated in the experiment. During 14 days they took the distilled water emulsion of cholesterol in the dose of 0.25 g of cholesterol per 1 kg of an animal. There was fast growth of cholesterol contents in the spleen homogenate. At the third day of the experiment the cholesterol contents increased till 460.0±18.0 mg%, and by the 14^th day of the experiment it was till 1201.0±131.2 mg%. The frozen sections were studied upon the activity of acid phosphomonoesterase by Gomori’s method at pH 6.2. It was found out that excessive admission of cholesterol into the spleen led to the growth of T-lymphocytes number around the artery of the lymphatic corpuscle and there was a sharp response to acid phosphomonoesterase. The marginal zone of the corpuscle increased. It had an intensive response to acid phosphomonoesterase at pH6.2 both in lymphocytes and in the reticular stromа of the spleen. The increase of the activity of acid phosphomonoesterase at hypercholesteremia in the spleen suggests the latter participates actively in the utilization of cholesterol in the organism.