THE ROLE OF NITRIC OXIDE AND NITRITE-ANION IN THE DEVELOPMENT OF ENDOTHELIAL DYSFUNCTION OF UMBILICAL CORD VESSELS IN THE EXACERBATION OF CYTOMEGALOVIRUS INFECTION IN THE THIRD TRIMESTER OF PREGNANCY
https://doi.org/10.36604/1998-5029-2019-74-92-97
Abstract
Aim. To study NO-synthase activity and nitrite-anion indices in umbilical vein endothelium in exacerbation of cytomegalovirus infection in the third trimester of pregnancy. Materials and methods. To study NO-synthase activity in the endothelium and the content of nitrite-anion in the umbilical cord homogenate obtained during childbirth at 38-39 weeks of pregnancy from 65 CMV-seropositive women with exacerbation of infection in the third trimester of pregnancy (the main group) and from 32 CMV-seronegative women (the control group). The material for the study was the endothelium of the umbilical cord vessels adjacent to the placenta. The content of nitrite anion in umbilical cord homogenate was studied by enzyme immunoassay. The activity of NO-synthase in the umbilical vein endothelium was studied by histochemical method followed by cytophotometric data processing. For morphological studies, semi-thin sections of umbilical vessels were used. Results. The study revealed 1.6 increase (p<0.001) in the activity of NO-synthase in the endothelium of the umbilical cord vein from CMV-seropositive women with exacerbation of infection in the third trimester of pregnancy compared to the group of CMV-seronegative women. The content of nitrite anion in the umbilical cord homogenate increased 4 times (p<0.001). Morphological examination revealed discomplexation and desquamation of endothelial cells, interstitial edema of the muscle layer, marginal standing and migration of leukocytes into the vascular wall and adjacent gelatin of Wharton. There was an expansion of the lumen of the umbilical cord veins. Conclusion. Based on the results of the study, it was shown that an increase in the activity of NO-synthase and the content of nitriteanion in the endothelium of the umbilical cord vein in exacerbation of CMV infection in the third trimester of pregnancy is associated with the development of inflammation, prolonging endothelial dysfunction, which can contribute to the obstruction of venous blood flow.
About the Author
N. N. Dorofienko
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
PhD (Med.), Senior Staff Scientist of Laboratory of Mechanisms of Etiopathogenesis and Recovery Processes of the Respiratory System at Non-Specific Lung Diseases
22 Kalinina Str., Blagoveshchensk, 675000, Russian Federation
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Review
For citations:
Dorofienko N.N. THE ROLE OF NITRIC OXIDE AND NITRITE-ANION IN THE DEVELOPMENT OF ENDOTHELIAL DYSFUNCTION OF UMBILICAL CORD VESSELS IN THE EXACERBATION OF CYTOMEGALOVIRUS INFECTION IN THE THIRD TRIMESTER OF PREGNANCY. Bulletin Physiology and Pathology of Respiration. 2019;(74):92-97. (In Russ.) https://doi.org/10.36604/1998-5029-2019-74-92-97
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