THE EXPRESSION OF CIRCUMFERENTIAL ENDOCANNABINOID SECOND TYPE RECEPTOR ON IMMUNOCOMPETENT CELLS WITH ACTIVATION OF IMMUNE SYSTEM IN VIVO

Abstract

The role of endogen cannabioid system in regulation of the immune response is discussed widely nowadays. So the expression of endocannabinoid receptor СВ₂ on immunocompetent cells with stimulation of immune system in vivo was studied. The expression of endocannabinoid receptor СВ₂ was performed by flow cytofluorometric method on flow cytometer using antibodies. The intracorporal irradiation of blood and the enrichment of blood with the ozone-oxygen mix were used for stimulation of the immune system. The immunostimulating effect of these physical methods is used for increasing of functional activity of immunocompetent cells through the activation of synthesis of cytokines and phagocytosis processes. This cytometric analysis of mononuclear leukocytes of healthy donors (28) demonstrated that the percentage of cells expressing the differentiating antigen to CB₂ receptor on their surface was more than 90% of all studied cells. Stimulation of immune system in vivo after intracorporal irradiation of blood leads to the suppression of quantity of immune cells which express CB₂ receptors on their surface for 30% (less than 60% of general quantity of immunocompetent cells). The amount of CB₂ receptor containing cells was not bigger than 70% after intravenous injection of ozone-oxygen mix. The obtained results of the study demonstrate that endocannabioid system is involved in the regulation of the immune response and can be used for direct influence on the inflammatory reaction of organism through CB₂ receptor.