GAS-TRANSPORT METABOLISM IN THE PERIPHERAL BLOOD AT GENERAL COOLING OF THE ORGANISM

Abstract

The aim of the research is to study in experimental conditions the character of changes of acid-base balance and bioenergetic processes in erythrocytes of the peripheral blood of rabbits at general cooling of the organism. It is established that as a result of daily cooling (from 15 till 30 days) of animals under experiment during three hours at temperature -30ºС pH of rabbits blood falls to 7.34±0.013 by the 30^th day of the experiment, and the quantity of pCO₂ in blood increases to 49.5 mm of mercury. The quantity of adenosinetriphosphoric acid in erythrocytes of the peripheral blood decreases to 0.34±0.06 mkmole/ml (control - 0.65±0.07 mkmole/ml), and the concentration of 2.3-biphosglycerate increases to 6.9 mkmole/ml. There is a drop of hemoglobin erythrocytes oxygenation to 96.0±3.2% (control - 99.5±3.0%), which leads to hypoxia in the organism of animals under experiment. Thus, the cooling of the organism suppresses energetic processes in erythrocytes of the peripheral blood and causes metabolic acidosis.