THE STUDY OF THE WAYS OF EXCRETION AND ACCUMULATION OF STAPHYLOCOCCAL ALPHA-TOXIN AT THE MODEL OF LUNG ABSCESS COMBINED WITH PURULENT BRONCHITIS IN THE EXPERIMENT WITH ANIMALS
Abstract
The aim of the research is the study of the ways of excretion and accumulation of staphylococcal alpha toxin from the lung abscess combined with purulent bronchitis. The methods of reproduction of these inflammations were developed during the experiment over 29 rabbits. As the source of purulent-necrotic process in lungs and bronchi in animals the mixture of Staphylococcus aureus meal (Wood-46 strain) was used in the combination with the stabilizer (Freund's adjuvant complete, USA), which was introduced into the lung tissue through the chest. To study the ways of the spread of bacterial toxin from purulent-necrotic nidi of lungs and bronchi, purified staphylococcal α-toxin tagged with I125 was introduced into the animals by the same method. It was found out that staphylococcal α-toxin tagged with I125 has the main ways of elimination into lymph and blood. Its maximal accumulation occurred in the lymph of the right lymphatic duct (23.9±1.5 units of relative specific activity) and it was twice as much as in the lymph of the chest lymphatic duct. The concentration of staphylococcal α-toxin in the blood was 10 times less than in the lymph. Staphylococcal α-toxin accumulates in big quantities in some vital organs: kidneys, lungs, liver, heart.
About the Authors
Vladimir P. Samsonov
Far Eastern Scientific Center of Physiology and Pathology of Respiration SB RAMS
Россия
Россия
Galina I. Chubenko
Amur State Medical Academy
Россия
Россия
Natalʼya V. Yusan
Amur State Medical Academy
Россия
Россия
Aleksey K. Samsonov
Amur State Medical Academy
Россия
Россия
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Review
For citations:
Samsonov V.P., Chubenko G.I., Yusan N.V., Samsonov A.K. THE STUDY OF THE WAYS OF EXCRETION AND ACCUMULATION OF STAPHYLOCOCCAL ALPHA-TOXIN AT THE MODEL OF LUNG ABSCESS COMBINED WITH PURULENT BRONCHITIS IN THE EXPERIMENT WITH ANIMALS. Bulletin Physiology and Pathology of Respiration. 2012;(46):77-80. (In Russ.)
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