USE OF EXTRACT CUSCUTA JAPONICA (CHOISY) TO STIMULATE THE GROWTH OF MYCOBACTERIUM TUBERCULOSIS
Abstract
The influence of the aqueous extract of Japanese Dodder (Cuscuta japonica) on the velocity of Mycobacterium tuberculosis growth has been shown. To accelerate the growth of Myc. tuberculosis, dry culture media of FAST-3L and of Lowenstein Jensen were mixed with the aqueous extract of Cuscuta japonica . It was found out that the complex chemical composition of the aqueous extract made of dry seeds or dry vegetative stems of the plant parasite Cuscuta japonica has a biological effect on Myc. tuberculosis. At mixing of standard culture media of FAST-3L and of Lowenstein Jensen with the aqueous extract of Cuscuta japonica , Myc. tuberculosis grow much faster preserving typical R-forms colonies. The mycobacterium in the first medium grow at the fifth day (2.8 times faster than in the standard culture medium), and in the second medium they grow at the 18th day (1.5 times faster than in the standard culture medium). The mechanism of Myc. tuberculosis growth acceleration demands a further study. The most probable is the fact that chemical substances of the aqueous extract of Cuscuta japonica directly or indirectly affect either the synthesis of cell wall components (peptidoglycan) or change its chemical composition. This leads to the access enhancement into the cell of trophic resources.
About the Authors
Viktor V. Katola
Institute of Geology and Nature Management of Far Eastern Branch RAS
Russian Federation
Russian Federation
Natalya Yu. Leusova
Institute of Geology and Nature Management of Far Eastern Branch RAS
Russian Federation
Russian Federation
References
1. Использование автоматизированной системы ВАСТЕС MGIT 960 в диагностике лекарственной устойчивости к резервным препаратам в г. Самаре / Я.М.Балабанова [и др.] // Туб. и бол. легких. 2009. №10. С.63–70.
2. Леусова Н.Ю., Некрасов Э.В. Использование видов рода повилика (Cuscuta L.) в медицине и биотехнологии // Бюл. физиол. и патол. дыхания. 2004. Вып.19. С.87–93.
3. Леусова Н.Ю. Особенности экологии растений-паразитов на примере повилики японской (Cuscuta japonica Choisy): автореф. дис. … канд. биол. наук. Благовещенск, 2006. 23 с.
4. Содержание минеральных элементов у растения- паразита CUSCUTA YAPONICA / Н.Ю.Леусова [и др.] // Пробл. региональной экологии. 2008. №3. С.68–71.
5. Питательная среда, ускоряющая рост микобактерий туберкулеза: пат. 2385926 МПК С12N 1/20 / авторы и заявители В.М.Катола, Н.Ю.Леусова; патентообладатель ИГиП ДВО РАН (RU).–№2007132045/13; заявл. 23.08.2007; опубл. 10.04. 2010, Бюл. №10.
6. Скотникова О.И. Молекулярно-биологические методы во фтизиатрии // Пробл. туб. и бол. легких. 2005. №8. С.5–10.
7. Роль ПЦР-анализа в комплексных бактериологических исследованиях во фтизиатрии / Л.Н.Черноусова [и др.] // Пробл. туб. и бол. легких. 2001. № 3. С.58–60.
8. Яковлев Г.П., Челомбитько В.А. Ботаника. Учебник для вузов. СПб: СпецЛит, СПХФА, 2001. 680 с.
9. Balabanova Ya.M., Fedorin I.M., Malomanova N.A., Drobnievskiy F., Nikolaevskiy V.V., Sun Kh., Mashkova Yu.A., Simak T.E., Kontsevaya I.S., Ignat'eva O.A., Mironova S.A. Tuberkulez i bolezni legkikh 2009; 10:63–70.
10. Leusova N.Yu., Nekrasov E.V. Bûlleten' fiziologii i patologii dyhaniyâ 2004; 19:87–93.
11. Leusova N.Yu. Osobennosti ekologii rasteniy-parazitov na primere poviliki yaponskoy (Cuscuta japonica Choisy): avtoreferat dissertatsii kandidata biologicheskikh nauk [The particularities of plants-parasite ecology at the example of Japanese dodder (Cuscuta japonica Choisy): abstract of thesis…candidate of biological sciences]. Blagoveshchensk; 2006.
12. Leusova N.Yu., Katola V.M., Radomskaya V.I., Radomskiy S.M. Problemy regional'noi ekologii 2008; 3:68–71.
13. Katola V.M., Leusova N.L. Patent 2385926 RF. Pitatel'naya sreda, uskoryayushchaya rost mikobakteriy tuberkuleza (Patent 2385926 RF. The medium, accelerating the growth of Mycobacterium tuberculosis); published 10.04. 2010.
14. Skotnikova O.I. Problemy tuberkuleza i bolezney legkikh 2005; 8:5–10.
15. Chernousova L.N., Larionova E. E., Sevast'yanova E.V., Golyshevskaya V.I. Problemy tuberkuleza i bolezney legkikh 2001; 3:58–60.
16. Yakovlev G.P., Chelombit'ko V.A. Botanika. Uchebnik dlya vuzov [Botany. The textbook for high schools]. St. Petersburg: SpetsLit, SPHFA; 2001.
Review
For citations:
Katola V.V., Leusova N.Yu. USE OF EXTRACT CUSCUTA JAPONICA (CHOISY) TO STIMULATE THE GROWTH OF MYCOBACTERIUM TUBERCULOSIS. Bulletin Physiology and Pathology of Respiration. 2012;(46):81-85. (In Russ.)
Views:
160
Email this article 