THE CHARACTERISTIC OF HEMOGLOBIN OXYGENATION IN ERYTHROCYTES OF PERIPHERAL BLOOD OF PREGNANT WOMEN AT CYTOMEGALOVIRUS INFECTION EXACERBATION DURING GESTATION

The work presents the data which reflect the state of processes of hemoglobin oxygenation and the reasons of their damage in erythrocytes of the peripheral blood of 50 pregnant women with the exacerbation of chronic cytomegalovirus infection (CMVI) at 20-22 weeks of gestation (the main group). The control group consisted of 20 healthy pregnant women at the same stages of gestation. Under biochemical research of erythrocytes of the peripheral blood of pregnant women of the main group oxygen-binding hemoglobin properties were found to be changed, which was revealed through the decrease of oxyhemoglobin concentration till 95.20±0.45% (р<0.05) at antibody titer of IgG to CMV 1:800 and till 90.15±0.35% (р<0.001 ) at antibody titer of IgG to CMV 1:1600 in comparison with the control group (98.00±0.65%). This fact was the result of the increased level of allosteric regulator of hemoglobin sensitivity to oxygen of 2.3-diphosphoglycerate in the main group till 5.96±0.4 mkmole/ml (р<0.01 ) at antibody titer of IgG to CMV 1:800 and till 6.7±0.07 mkmole/ml (р<0.001 ) at antibody titer of IgG to CMV 1:1600 (in the control group it was 4.90±0.15 mkmole/ml). In the peripheral blood of pregnant women of the main group there was also an accumulation of non-organic anions decreasing pH of the blood till 7.32±0.03 (р<0.05) at antibody titer of IgG CMV 1:800 and till 7.25±0.02 (р<0.01) at antibody titer of IgG to CMV 1:1600 (in the control it was 7.40±0.07), which increased the probability of the formation of heme complex with 2.3-diphosphoglycerate and worsened the binding of hemoglobin with oxygen. Against this background there was the growth of methemoglobin till 0.90±0.03% (р<0.05) at antibody titer of IgG to CMV 1:800 and till 1.50±0.04% (р<0.001) at antibody titer of IgG to CMV 1:1600 (in the control group it was 0.60±0.015%) and the decrease of an antioxidant function of glutathione peroxidase till 9.60±0.13 Un/rHb (р<0.01) at antibody titer of IgG to CMV 1:800 and till 6.50±0.15 Un/гHb (р<0.001) at antibody titer of IgG to CMV 1:1600 (in the control group it was 15.70±0.17 Un/гHb). The obtained data show an important role of active CMVI in the development of disturbances of hemoglobin oxygenation processes and mechanisms of their regulation in erythrocytes of the peripheral blood of pregnant women, which could result in the formation of hemic hypoxia decreasing the transportation of oxygen into the fetus blood.

оксигемоглобин, 2,3-дифосфоглицерат, цитомегаловирус, гестация, oxyhemoglobin, 2.3-diphosphoglycerate, cytomegalovirus, gestation period

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