DIAGNOSTIC RESULTS OF INTRAUTERINE AND PERINATAL INFECTIONS IN PREMATURE LOW-WEIGHT CHILDREN

The aim of the study was to evaluate of up-to-date opportunities to diagnose intrauterine and perinatal infections in premature children. Seventy-eight premature low-weight newborns, including those with low and extremely low birth weight, and their mothers were examined. Methods used: molecular diagnostics (PCR) to identify genetic material of perinatally significant bacterial and viral agents, and serological assay (ELISA) to identify serological markers of active stages of Herpes virus (Herpes simplex virus, Cytomegalovirus) and Chlamydial infections. A group of mothers of low-weight children was found to consist mainly of women with compromised obstetric history and signs of placental insufficiency probably due to intrauterine infections. PCR investigation of placental tissue specimens revealed nucleic acids of bacterial and viral agents in 73.5% of women. Morphological examination of infected placentas revealed inflammatory changes (placentites, choriodeciduites, villusites) in 100% of cases, and different abnormalities (improperly attached cord, disorders of implantation and placentation) in 46.1% of cases. Upon examination of clinical specimens of newborns, markers of perinatally significant infections were diagnosed in 41.0% of cases. Most frequently found were Cytomegalovirus (14.1%) and Ureaplasma spp. (17.9%). Upon serological examination, no markers of active stages of the given infections were found. To increase efficiency of diagnostics of intrauterine infections in a newborn, it is important to take the maximum possible number of tests of body fluids by PCR method. Intrauterine infection is diagnosed using the entire set of clinical, laboratory and morphological data with account for the time of examination (optimal time: first days of life).

маловесные недоношенные дети, внутриутробные инфекции, перинатальные инфекции, диагностика, premature low-weight newborns, intrauterine infections, perinatal infections, diagnostics

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