EFFECT OF THE COMPLEX MEDICATION MADE OF BEE ROYAL JELLY AND PROPOLIS AT PULMONARY EDEMA OF RATS

The purpose of this study was to investigate the mechanisms of effect of inhaled mixture made on the basis of bee royal jelly and propolis (apingalin) in experimental pulmonary edema. Studies were conducted on adult rats (male) with the weight of 0.18-0.2 kg; the rats were divided into groups: intact animals, control animals with adrenal experimental pulmonary edema, experience animals with pulmonary edema; all of them then inhaled apingalin. The bodies for morphological and biochemical analyses were collected one day after the termination of the experiment. In preparations of isolated trachea of rats, the reaction of constriction-relaxation after adding apingalin was studied. When studying the therapeutic effect of the mixture, the weight ratio and dry residue were measured; the histological examination of lung tissue was done. The activity of the process of lipid peroxidation was assessed by the content of diene, triene conjugates and Schiff bases in lung homogenates; oxidized protein modification was estimated in terms of carbonyl derivatives. It was found that the effect of the drug on the basis of royal jelly and bee propolis on isolated tracheal preparation is biphasic: the first one is a short-term phase of constriction of the preparation, and the second one is a long-term phase of its relaxation mediated through the activation of beta-adrenergic receptors. Course of 10-day inhalation of apingalin reduces the amount of oxidized forms of the protein and prevents the oxidation of lipids in the lung tissue of rats.

маточное молочко пчел, прополис, отек легких, перекисное окисление липидов, окислительная модификация белка, bee royal jelly, propolis, pulmonary edema, lipid peroxidation, oxidative modification of proteins

1. Teoriya i sredstva apiterapii / V.N.Krylov [i dr.]. M.: Komil'fo, 2007. 296 s.

2. Flavonoidy: biokhimiya, biofizika, meditsina / Yu.S.Tarakhovskii [i dr.]. Pushchino: Synchrobook, 2013. 310 s.

3. Duration-dependent hepatoprotective effects of Propolis extract against carbon tetrachloride-induced acute liver damage in rats / M.Bhadauria [et al.] // Adv. Ther. 2007. Vol.24, №5. P.1136-1145.

4. Cavuşoğlu K., Yapar K., Yalçin E. Royal jelly (honey bee) is a potential antioxidant against cadmium-induced genotoxicity and oxidative stress in albino mice // J. Med. Food. 2009. Vol.12, №6. P.1286-1292.

5. Glantz S. Primer of Biostatistics. 6th Edition. New York: McGraw Hill Professional, 2010. 520 p.

6. Royal jelly supplementation improves lipoprotein metabolism in humans / H.Guo [et al.] // J. Nutr. Sci. Vitaminol. (Tokyo). 2007. Vol.53, №4. P.345-348.

7. Guo H., Kouzuma Y., Yonekura M. Structures and properties of antioxidative peptides derived from royal jelly protein // Food Chem. 2009. Vol.113. P.238-245.