BASOPHIL ACTIVATION TEST IN VITRO DIAGNOSIS OF HYPERSENSITIVITY TO BETA-LACTAM ANTIBIOTICS IN PATIENTS WITH BRONCHIAL ASTHMA

Abstract

Using flow cytometry the spectrum and prevalence of sensitization to beta-lactam antibiotics in 76 patients with bronchial asthma of allergic, not allergic and mixed type were estimated and the frequency of cross-responses to penicillin was found out. In 38 patients at the time of the study moderate bronchial asthma was diagnosed and in 38 patients there was a severe course of the disease. Dosage history intolerance was observed in patients with mixed and allergic type of the disease. It was shown that the sensitization of patients with asthma was significantly higher (p<0.05) than in healthy people in the control group and was identified for penicillins, cephalosporins of I and II generation and carbapenems. Significantly more frequent it was in the group with mixed asthma (р<0.05). Sensitization to penicillin was found in 12 patients with bronchial asthma. Three patients were identified to have monosensibilization to penicillin, and 9 patients had besides penicillin sensitization to other groups of beta-lactam antibiotics. Cross reactions of penicillin with carbapenems (50%) and with cephalosporins of I generation (25%) were identified most often.