ROLE OF NF-KВ AND CYTOKINES IN THE FORMATION OF PLACENTAL INSUFFICIENCY IN OBESE WOMEN WITH HYPOTHALAMIC DYSFUNCTION

Abstract

The role of nuclear factor kappa B (NF-kB) and cytokines in the formation of placental insufficiency in 64 pregnant women with obesity and hypothalamic dysfunction (the main group) was studied. 30 relatively healthy women made the control group. The level of TNF-α, IL-1β and IL-10 were defined in the homogenate of placenta (38-40 weeks). In all cases the morphological study of placenta was done with electronic-microscopic and histological methods. The pregnant women of the main group in comparison with the control one were revealed to have the increase of TNF-α till 68.7±0.79 pg/mL (p=0.007) and of IL-1β till 93.7±0.92 pg/mL (p=0.021) in the homogenate of placenta, the reduction of IL-10 till 19.7±1.02 pg/ml (p=0.000), against 1.2 times increase in the activity of NF-kB factor (р=0.007), which was accompanied by a violation of maturation of chorionic villi, involutive and degenerative changes in the structure of the chorion, microcirculatory disturbances. The obtained results indicate that the development of placental insufficiency in obese women with hypothalamic dysfunction are associated with immune changes caused by the activation of NF-kB followed by the release of cytokines which influence the processes of angiogenesis, coagulation and apoptosis in placenta, with the formation of the primary and under the failure of compensatory processes chronic placental insufficiency.