Cell therapy in treatment of skin burns
https://doi.org/10.36604/1998-5029-2020-77-117-124
Abstract
The review summarizes the results of modern research in the field of cell therapy for skin burns. The relevance of conducting these studies both in Russia and in the world is described. The methods of influence on the skin regeneration after burns in addition to cell therapy are indicated. A history reference on the development of cell therapy for burn skin lesions is given. The documents governing the conduct of cell therapy in Russia are presented. The advantages and disadvantages of cell technology using keratinocytes are described. The methods of cell therapy for skin burns using fibroblasts are analyzed. The role of three-dimensional tissue-engineered structures – scaffolds in the regeneration of the skin is described. Their classification is given by the duration of wound coverage (permanent, semi-permanent and temporary), by composition (cellular, acellular), by type of material (synthetic, biological, which are divided into allogeneic and autologous). The main representatives of each group that are used in research as therapy in the treatment of skin burns are described: Biobrane, Integra, Dermagraft, TransCyte, Hyalograft 3D, Laser skin epidermal replacement, TissueTech autograft system. The data on the experimental testing of each of the representatives are presented. The issue of improving vascularization of tissue-engineering structures using bioreactors was also raised. According to the results of the review, it was concluded that the use of three-dimensional structures in the treatment of burn skin lesions shows the greatest efficacy and safety among pronounced cell therapy options in clinical practice. At the same time, the existing drawbacks of the analyzed samples require further study and analysis.
About the Authors
A. A. Yatsenko
Amur State Medical Academy;
Scientific Research Center “IQ Biofabric"
Russian Federation
Anton A. Yatsenko, MD, Pathologist, Postgraduate Student
Russian Federation
Anton A. Yatsenko, MD, Pathologist, Postgraduate Student
S. V. Barannikov
Amur State Medical Academy
Russian Federation
Sergey V. Barannikov, 6 th year Student of Medical Faculty
Russian Federation
Sergey V. Barannikov, 6 th year Student of Medical Faculty
I. Yu. Makarov
Amur State Medical Academy
Russian Federation
Igor Yu. Makarov, MD, PhD, D.Sc. (Med.), Professor, Head of Department of Pathology
Russian Federation
Igor Yu. Makarov, MD, PhD, D.Sc. (Med.), Professor, Head of Department of Pathology
I. V. Borozda
Amur State Medical Academy
Russian Federation
Ivan V. Borozda, MD, PhD, D.Sc. (Med.), Professor, Head of Department of Traumatology
Russian Federation
Ivan V. Borozda, MD, PhD, D.Sc. (Med.), Professor, Head of Department of Traumatology
Yu. A. Spirina
Amur State Medical Academy
Russian Federation
Yulia A. Spirina, 3rd year Student of Medical Faculty
Russian Federation
Yulia A. Spirina, 3rd year Student of Medical Faculty
References
1. Evdokimov V.I., Kourov A.S. Genesis of research on burn injury (analysis of domestic articles in 2005–2017). Mediko-biologičeskie i socialʹno-psihologičeskie problemy bezopasnosti v črezvyčajnyh situaciâh = Medicо-Biological and Socio-Psychological Problems of Safety in Emergency Situations 2018; (4):108–120 (in Russian).
2. https://doi.org/10.25016/2541-7487-2018-0-4-108-120
3. Olifirova O.S., Kozka A.A. Optimization techniques to treat injuries of different genesis. Pacific Medical Journal 2019; (2):80–83 (in Russian). https://doi.org/10.17238/PmJ1609-1175.2019.2.80-83
4. Olifirova O.S., Kozka A.A., Volosenkova E.A. Possibilities of therapy optimization of patients with superficial burning wounds. Аmurskiy meditsinskiy zhurnal = Amur Medical Journal 2017; (3):140–141. doi: 10.22448/AMJ.2017.3.140-141
5. Metcalfe A.D., Ferguson M.W.J. Bioengineering skin using mechanisms of regeneration and repair. Biomaterials 2007; 28(34):5100–5113. doi: 10.1016/j.biomaterials.2007.07.031
6. Groeber F., Holeiter M., Hampel M., Hinderer S., Schenke-Layland K. Skin tissue engineering – in vivo and in vitro applications. Adv. Drug Deliv. Rev. 2011; 63(4-5):352–366. doi: 10.1016/j.addr.2011.01.005
7. Gantwerker E.A., Hom D.B. Skin: histology and physiology of wound healing. Clin. Plast. Surg. 2012; 39(1):85–97. doi: 10.1016/j.cps.2011.09.005
8. Gallico G.G.3rd, O'Connor N.E, Compton C.C., Kehinde O., Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. New Engl. J. Med. 1984; 311(7):448–451. doi:10.1056/NEJM198408163110706
9. Potten C.S, Booth C. Keratinocyte stem cells: a commentary. J. Invest. Dermatol. 2002; 119(4):888–899. doi: https://doi.org/10.1046/j.1523-1747.2002.00020.x
10. Ronfard V., Rives J.M., Neveux Y., Carsin H., Barrandon Y. Long-term regeneration of human epidermis on third degree burns transplanted with autologous cultured epithelium grown on a fibrin matrix. Transplantation 2000; 70(11):1588–1598. doi: 10.1097/00007890-200012150-00009
11. Sood R., Roggy D., Zieger M., Balledux J., Chaudhari S., Koumanis D.J., Mir H.S., Cohen A., Knipe C., Gabehart K., Coleman J.J. Cultured epithelial autografts for coverage of large burn wounds in eighty-eight patients: the Indiana University experience. J. Burn Care Res. 2010; 31(4):559–568. doi: 10.1097/BCR.0b013e3181e4ca29
12. Billingham R.E., Reynolds J. Transplantation studies on sheets of pure epidermal epithelium and on epidermal cell suspensions. Br. J. Plast. Surg. 1952; 5(1):25–36. doi: 10.1016/s0007-1226(52)80004-9
13. Schlabe J., Johnen C., Schwartlander R., Moser V., Hartmann B., Gerlach J.C., Küntscher M.V. Isolation and culture of different epidermal and dermal cell types from human scalp suitable for the development of a therapeutical cell spray. Burns 2008; 34(3):376–384. doi: 10.1016/j.burns.2007.04.005
14. Svensjö T., Yao F., Pomahac B., Eriksson E. Autologous keratinocyte suspensions accelerate epidermal wound healing in pigs. J. Surg. Res. 2001; 99(2):211–221. doi: 10.1006/jsre.2001.6197
15. Kopp J., Jeschke M.G., Bach A.D., Kneser U., Horch R.E. Applied tissue engineering in the closure of severe burns and chronic wounds using cultured human autologous keratinocytes in a natural fibrin matrix. Cell Tissue Bank. 2004; 5(2):89–96. doi: 10.1023/B:CATB.0000034082.29214.3d
16. Eldardiri M., Martin Y., Roxburgh J., Lawrence-Watt D.J., Sharpe J.R. Wound contraction is significantly reduced by the use of microcarriers to deliver keratinocytes and fibroblasts in an in vivo pig model of wound repair and regeneration. Tissue Eng. Part A 2012; 18(5-6):587–597. doi: 10.1089/ten.TEA.2011.0258
17. Nolte S.V., Xu W., Rennekampff H.O., Rodemann H.P. Diversity of Fibroblasts – A Review on Implications for Skin Tissue Engineering. Cells Tissues Organs 2008 187(3):165–176. doi: 10.1159/000111805
18. Wong T., McGrath J.A., Navsaria H. The Role of Fibroblasts in Tissue Engineering and Regeneration. Br. J. Dermatol. 2007; 56(6):1149–1155. doi: 10.1111/j.1365-2133.2007.07914.x
19. Spiekstra S.W., Breetveld M., Rustemeyer T., Scheper R.J., Gibbs S. Wound-healing factors secreted by epidermal keratinocytes and dermal fibroblasts in skin substitutes. Wound Repair Regen. 2007; 15(5):708–717. doi: 10.1111/j.1524-475X.2007.00280.x
20. Yamakawa S., Hayashida K. Advances in surgical applications of growth factors for wound healing. Burns Trauma 2019; 7:10. doi: 10.1186/s41038-019-0148-1
21. Fedorov V.D., Sarkisov D.S., Аlekseev А.А., Тumanov V.P., Serov G.G. The use of cultured fibroblasts for skin burns. Vrach 1999; 11:26–28 (in Russian).
22. Vozdvizhensky V.I., Budkevich L.I., Shurova L.V. Organization of staged medical care for young children with thermal injury. Proceedings of the VII All-Russian Scientific and Practical Conference on the Problem of Thermal Damage. Chelyabinsk; 1999 (in Russian).
23. Horst B., Chouhan G., Moiemen N.S., Grover L.M. Advances in keratinocyte delivery in burn wound care. Adv. Drug Deliv. Rev. 2018; 123:18–32. doi: 10.1016/j.addr.2017.06.012
24. Wagner D.O., Zinoviev E.V., Krylov K.M., Krylov P.K., Soloshenko V.V., Kostyakov D.V., Yurkevich Yu.V., Enukashvily N.I., Blinova M.I., Aleksandrova O.I., Mikhailova N.A. Experience in the clinical use of allogeneic fibroblasts in patients with severe burns. Herald of North-Western State Medical University named after I.I. Mechnikov. 2018; 10(3):65–72 (in Russian). doi: 10.17816/mechnikov201810365-72
25. Lykov A.P., Poveshchenko O.V., Bondarenko N.A., Surovtseva M.A. Therapeutic potential of fibroblast combined with platelet-rich plasma on burn skin wound healing. J. Biol. Today's World 2019; 8(1):1–5 doi: 10.15412/J.JBTW.01070201
26. Shevchenko R.V., James S.L., James S.E. A review of tissue-engineered skin bioconstructs available for skin reconstruction. J. R. Soc. Interface 2010; 7(43):229–258. doi: 10.1098/rsif.2009.0403
27. Shakespeare P.G. The role of skin substitutes in the treatment of burn injuries. Clin. Dermatol. 2005; 23(4):413–418. doi: 10.1016/j.clindermatol.2004.07.015
28. Melkun E.T., Few J.W. The use of biosynthetic skin substitute (Biobrane) for axillary reconstruction after surgical excision for hidradenitis suppurativa. Plast. Reconstr. Surg. 2005; 115(5):1385–1388. doi: 10.1097/01.prs.0000157013.40191.91
29. Supp D.M., Boyce S.T. Engineered skin substitutes: practices and potentials. Clin. Dermatol. 2005; 23(4):403–412. doi: 10.1016/j.clindermatol.2004.07.023
30. Bello Y.M., Falabella A.F., Eaglstein W.H. Tissue-engineered skin. Current status in wound healing. Am. J. Clin. Dermatol. 2001; 2(5):305–313. doi: 10.2174/1381612823666170526094606
31. Dodson B.P., Levine A.D. Challenges in the translation and commercialization of cell therapies. BMC Biotechnol. 2015; 15:70. doi: 10.1186/s12896-015-0190-4
32. Dickinson L.E., Gerecht S. Engineered biopolymeric scaffolds for chronic wound healing. Front. Physiol. 2016; 7:341. doi: 10.3389/fphys.2016.00341
33. Raguse J.D., Gath H.J. The buccal fad pad lined with a metabolic active dermal replacement (dermagraft) for treatment of defects of the buccal plane. Br. J. Plast. Surg. 2004; 57(8):764–768. doi: 10.1016/j.bjps.2004.05.003
34. Lovett M., Lee K., Edwards A., Kaplan D.L. Vascularization strategies for tissue engineering. Tissue Eng. Part B Rev. 2009; 15(3):353–370. doi: 10.1089/ten.TEB.2009.0085
35. Limova M. Active wound coverings: bioengineered skin and dermal substitutes. Surg. Clin. North Am. 2010; 90(6):1237–1255. doi: 10.1016/j.suc.2010.08.004
36. Uccioli L. A clinical investigation on the characteristics and outcomes of treating chronic lower extremity wounds using the tissuetech autograft system. Int. J. Low. Extrem. Wounds 2003; 2(3):140–151. doi: 10.1177/1534734603258480
37. MacNeil S. Progress and opportunities for tissue-engineered skin. Nature 2007; 445(7130):874–880. doi: 10.1038/nature05664
Review
For citations:
Yatsenko A.A., Barannikov S.V., Makarov I.Yu., Borozda I.V., Spirina Yu.A. Cell therapy in treatment of skin burns. Bulletin Physiology and Pathology of Respiration. 2020;(77):117-124. (In Russ.) https://doi.org/10.36604/1998-5029-2020-77-117-124
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