Detection of chronic obstructive pulmonary disease biomarkers using ROC-analysis
https://doi.org/10.36604/1998-5029-2022-85-32-36
Abstract
Aim. To identify key diagnostic criteria for chronic obstructive pulmonary disease (COPD).
Materials and methods. We examined 112 patients with mild, moderate and severe COPD and 32 relatively healthy volunteers with no disturbances in the lung function. The level of IL-4, IL-6, IL-10, IL-17A, TNF-α, IFN-γ, TGF-β1 and CD4+CD126+ cells was determined by means of flow cytometry method. Prooxidant and antioxidant markers of oxidative stress 8-OHdeoxyguanosine, protein carbonyl, glutathione, glutathione reductase, glutathione peroxidase, thioredoxin-1, and thioredoxin reductase-1 were assessed by enzyme immunoassay.
Results. The presence of high-ranking correlations of the proinflammatory link of immunity and components of the prooxidant system was revealed, which indicates the presence of systemic alterative processes in patients with COPD. Based on ROC-analysis, key biomarkers were identified in patients with stable COPD: 8-OH-deoxyguanosine, CD4+CD126+ cells, IL-17, and thioredoxin, and their threshold levels were established.
Conclusion. The recommended diagnostic criteria will optimize the therapeutic strategies for COPD.
About the Authors
T. I. Vitkina
Vladivostok Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Medical Climatology and Rehabilitative Treatment
Russian Federation
Russian Federation
Tatiana I. Vitkina, PhD, DSc (Biol.), Professor of RAS, Head of Laboratory of Medical Ecology and Recreational Resources
73g Russkaya Str., Vladivostok, 690105
E. V. Kondratyeva
Vladivostok Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Medical Climatology and Rehabilitative Treatment
Russian Federation
Russian Federation
Elena V. Kondratyeva, PhD (Biol.), Staff Scientist, Laboratory of Medical Ecology and Recreational Resources
73g Russkaya Str., Vladivostok, 690105
References
1. Anaev E.Kh. [Biological markers of chronic obstructive pulmonary disease]. Prakticheskaya pul'monologiya 2018; (1):26‒32 (in Russian).
2. Barnes P.J. Inflammatory mechanisms in patients with chronic obstructive pulmonary disease. J. Allergy Clin. Immunol. 2016; 138(1):16‒27. https://doi.org/10.1016/j.jaci.2016.05.011
3. Ni L., Dong Ch. Roles of myeloid and lymphoid cells in the pathogenesis of chronic obstructive pulmonary disease. Front. Immunol. 2018; 9: 1431. https://doi.org/10.3389/fimmu.2018.01431
4. Boukhenouna S., Wilson A., Bahmed K., Kosmider B. Reactive oxygen species in chronic obstructive pulmonary disease. Oxid. Med. Cell. Longev. 2018: 5730395. https://doi.org/10.1155/2018/5730395
5. Nurwidya F., Damayanti T., Yunus F. The role of innate and adaptive immune cells in the immunopathogenesis of chronic obstructive pulmonary disease. Tuberc. Respir. Dis. (Seoul) 2016; 79(1):5–13. https://doi.org/10.4046/trd.2016.79.1.5
6. Otasevic V., Vucetic M., Grigorov I., Martinovic V., Stancic A. Ferroptosis in different pathological contexts seen through the eyes of mitochondria. Oxid. Med. Cell. Longev. 2021: 5537330. https://doi.org/10.1155/2021/5537330
7. Rahman I., Adcock I. M. Oxidative stress and redox regulation of lung inflammation in COPD. Eur. Respir. J. 2006; 28:219‒242. https://doi.org/10.1183/09031936.06.00053805
8. Antus B., Kardos Z. Oxidative stress in COPD: molecular background and clinical monitoring. Curr. Med. Chem. 2015; 22(5):627‒650. https://doi.org/10.2174/092986732205150112104411
9. Mahmood D.F.D., Abderrazak A., El Hadri K., Simmet T., Rouis M. The thioredoxin system as a therapeutic target in human health and disease. Antioxid. Redox Signal. 2013; 19(11):1266–1303. https://doi.org/10.1089/ars.2012.4757
10. El Hadri K., Smith R., Duplus E., El Amri C. Inflammation, oxidative stress, senescence in atherosclerosis: Thioredoxine-1 as an emerging therapeutic target. Int. J. Mol. Sci. 2022; 23(1):77. https://doi.org/10.3390/ijms23010077
11. Mohamed I.N., Li L., Ismael S., Ishrat T., El-Remessy A.B. Thioredoxin interacting protein, a key molecular switch between oxidative stress and sterile inflammation in cellular response. World J. Diabetes 2021; 12(12):1979‒1999. https://doi.org/10.4239/wjd.v12.i12.1979
12. Pichavant M., Rémy G., Bekaert S., Le Rouzic O., Kervoaze G., Vilain E., Just N., Tillie-Leblond I., Trottein F., Cataldo D., Gosset P. Oxidative stress-mediated iNKT-cell activation is involved in COPD pathogenesis. Mucosal Immunol. 2014; 7(3):568–578. https://doi.org/10.1038/mi.2013.75
13. Rossman M.J., Groot H.J., Reese V., Zhao J., Amann M., Richardson R.S. Oxidative stress and COPD: The effect of oral antioxidants on skeletal muscle fatigue. Med. Sci. Sports Exerc. 2013; 45(7):1235–1243. https://doi.org/10.1249/MSS.0b013e3182846d7e
14. Eeden S., Sin D.D. Oxidative stress in chronic obstructive pulmonary disease: A lung and systemic process. Can. Respir. J. 2013; 20(1):27–29. https://doi.org/10.1155/2013/509130
Review
For citations:
Vitkina T.I., Kondratyeva E.V. Detection of chronic obstructive pulmonary disease biomarkers using ROC-analysis. Bulletin Physiology and Pathology of Respiration. 2022;(85):32-36. (In Russ.) https://doi.org/10.36604/1998-5029-2022-85-32-36
Views:
245
Email this article 