Pro-inflammatory activity of COPD macrophages in the in vitro experiment
https://doi.org/10.36604/1998-5029-2023-88-17-26
Abstract
Introduction. Chronic obstructive pulmonary disease (COPD) is a severe, progressive disease characterized by irreversible airway obstruction and emphysema. Prolonged exposition to airborne toxicants triggers irreversible processes leading to aberrant polarization of macrophages and defective phagocytosis, imbalance of pro- and anti-inflammatory cytokines.
Aim. To study the features of the reaction of macrophages in COPD patients to the action of pro- and anti-inflammatory stimuli.
Materials and methods. The study included 8 COPD patients and 6 control subjects. All persons underwent clinical and functional examination and sampling of peripheral venous blood for the isolation of monocytes. Cells had been cultured with 50 ng/mL granulocyte-macrophage colony-stimulating factor for 6 days, and then were polarized into pro-inflammatory (M1) and anti-inflammatory (M2) macrophages by adding E. coli lipopolysaccharides (LPS) 100 ng/mL and recombinant human interferon gamma (IFN-γ) 20 ng/ml, or interleukin 4 (IL-4) 20 ng/ml, respectively. Cytokine analysis was performed in the culture medium supernatant by multiplex analysis on a flow cytometer.
Results. In the non-polarized state (M0), cells of COPD patients and the control group did not differ in the rate of cytokine production. At the same time, under LPS/IFN-γ stimulation a more pronounced increase in pro-inflammatory CXCL10 was observed in patients with COPD as compared with the control group (104.5-fold vs. 41.6-fold, p=0.04), and in the control group, on the contrary, the production of anti-inflammatory IL-10 was increased to a greater extent (99.6-fold vs. 30.5- fold, p=0.06). The effect of IL-4 on COPD macrophages was accompanied by a more pronounced decrease in IL-6, TNFα and IL-8 as compared to the group of healthy subjects.
Conclusion. COPD macrophages are characterized by increased sensitivity to polarizing stimuli: under M1 stimulation we observed increased pro-inflammatory activity and under conditions of M2 differentiation, on the contrary, more pronounced inhibition of pro-inflammatory mediators occurred.
About the Authors
I. Yu. Sugaylo
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Ivana Yu. Sugaylo, PhD (Med.), Junior Staff Scientist, Laboratory of Molecular and Translational Research
22 Kalinina Str., Blagoveshchensk, 675000
D. E. Naumov
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Denis E. Naumov, PhD (Med.), Head of Laboratory of Molecular and Translational Research
22 Kalinina Str., Blagoveshchensk, 675000
D. A. Gassan
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Dina A. Gassan, PhD (Med.), Staff Scientist, Laboratory of Molecular and Translational Research
22 Kalinina Str., Blagoveshchensk, 675000
O. O. Kotova
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Olesya O. Kotova, PhD (Med.), Junior Staff Scientist, Laboratory of Molecular and Translational Research
22 Kalinina Str., Blagoveshchensk, 675000
Y. G. Gorchakova
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Yana G. Gorchakova, Research Laboratory Assistant, Laboratory of Molecular and Translational Research
22 Kalinina Str., Blagoveshchensk, 675000
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Review
For citations:
Sugaylo I.Yu., Naumov D.E., Gassan D.A., Kotova O.O., Gorchakova Y.G. Pro-inflammatory activity of COPD macrophages in the in vitro experiment. Bulletin Physiology and Pathology of Respiration. 2023;(88):17-26. (In Russ.) https://doi.org/10.36604/1998-5029-2023-88-17-26
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