COMPOSITION OF FATTY ACIDS AND THE LEVEL OF THEIR METABOLITES AT PARTIALLY CONTROLLED BRONCHIAL ASTHMA AGAINST THE BACKGROUND OF INTEGRATED TREATMENT WITH THE USE OF MILLIMETER THERAPY

To assess the anti-inflammatory effect of millimeter therapy in the treatment of partially controlled asthma, the fatty acid composition of erythrocyte membranes and their oxygenase derivatives were studied. Eighty-five subjects aged 18 to 45 years old were examined, 60 of them were diagnosed with partially controlled asthma and 25 were a control group, which consisted of conditionally healthy subjects without bronchopulmonary pathology. Two study groups were formed. Patients of the 1^st group (n=30) received only basic medical treatment: inhalation glucocorticosteroid and β₂-agonists of short action on demand. Patients of the 2^nd group (n=30) underwent complex treatment: basic medical treatment in combination with millimeter therapy according to the developed technique. Clinical symptoms (frequency of nocturnal and daily asthma attacks per week, severity of cough, need for short-acting bronchodilators during the day), results of the asthma symptom control questionnaire (ACQ-5 test), lung function, composition of fatty acid (FA) in erythrocyte membranes, level of eicosanoids were assessed. The dynamics of clinical-functional and laboratory indicators in patients was evaluated one month after the start of treatment. The study established a modification of FA erythrocyte membranes composition, characterized by an increase in the proportion of some representatives of saturated and monounsaturated FA, increased production of arachidonic acid and its cyclooxygenase and lipoxygenase metabolites (thromboxane B₂, leukotriene B₄) against the background of deficiency of eicosapentaenoic and docosapentaenoic acids. A significant decrease in the level of oxygenated derivatives of fatty acids in the blood plasma of patients was revealed against the background of positive dynamics of clinical course of the disease in the 2^nd group receiving complex treatment, which indicates the normalization of metabolic transformations of oxygenated derivatives of fatty acids, and as a consequence the extinction of allergic inflammation under the influence of electromagnetic waves of the millimeter range of extremely high frequency.

bronchial asthma, fatty acids, eicosanoids, millimeter therapy

1. Банержи А. Медицинская статистика понятным языком: вводный курс (пер. с англ.). М: Практическая медицина, 2007. 287 с.

2. Гвозденко Т.А., Антонюк М.В., Фотина О.Н., Степанова О.П. Применение КВЧ терапии в комплексной реабилитации больных бронхиальной астмой: медицинская технология. Владивосток, 2015. 15 с.

3. Гржибовский А.М. Типы данных, проверка распределения и описательная статистика // Экология человека. 2008. №1. С.52-58.

4. Гржибовский А.М. Анализ количественных данных для двух независимых групп // Экология человека. 2008. №2. С.54-61.

5. Денисенко Ю.К., Новгородцева Т.П., Лобанова Е.Г., Калинина Е.П. Состав жирных кислот клеток крови в условии системного воспаления при хронических заболеваниях органов дыхания // Национальная Ассоциация ученых. 2015. №5-4(10). С.26-29.

6. Реброва О.Ю. Статистический анализ медицинских данных. Применение пакета прикладных программ STATISTICA. М.: Медиа Сфера, 2002. 312 с.

7. Ходосова К.К., Гвозденко Т.А., Антонюк М.В., Минеева Е.Е., Юренко А.В., Переломова О.В. Влияние КВЧ-терапии на состояние цитокинового статуса у больных частично контролируемой бронхиальной астмой // Здоровье. Медицинская экология. Наука. 2017. №4(71). С.63-68. http://doi.org/10.5281/zenodo.835326

8. Bligh E.C., Dyer W.J.A rapid method of total lipid extraction and purification // Can. J. Biochem. Physiol. 1959. Vol.37, №8. Р.911-917. doi: 10.1139/o59-099

9. Carreau J.P., Dubacq J.P. Adaptation of a macro-scale method to the micro-scale for fatty acid methyl transesterification of biological lipid extract // J. Chromatogr. 1978. Vol.151, Iss.3. Р.384-390. https://doi.org/10.1016/S0021-9673(00)88356-9

10. Giudetti A.M., Cagnazzo R. Beneficial effects of n-3 PUFA on chronic airway inflammatory diseases // Prostaglandins Other Lipid Mediat. 2012. Vol.99, №3-4. Р.57-67. doi: 10.1016/j.prostaglandins.2012.09.006

11. Global Initiative for Asthma (GINA). Global strategy for asthma management and prevention (Update 2016). URL: http://ginasthma.com.

12. Novgorodtseva T.P., Denisenko Yu.K., Zhukova N.V., Antonyuk M.V., Knyshova V.V., Gvozdenko T.A. Modification of the fatty acid composition of the erythrocyte membrane in patients with chronic respiratory diseases // Lipids in Health and Disease. 2013. Vol.12. P.117.