The study of morphological and biodegradable properties of porous scaffold of gelatin for use in tissue engineering of lung

The use of collagen or gelatin-based scaffolds mimicking the in vivo conditions of the microenvironment is beginning to be used more widely. This is facilitated by the deepening of the understanding of cell-cell interactions in vivo, which allows to artificially form a his-toarchitecture suitable for cells and the microenvironment of the scaffold. Gelatin is a collagen derivative. This is one of the most promising and inexpensive materials for creating scaffolds. The purpose of the study was to investigate the morphological, biodegradable and antibacterial properties, as well as the rate of scaffold degradation in vivo and in vitro of glu-taraldehyde-crosslinked gelatin polymer modified by dihydroquercetin and arabinogalactan. The study of the morphological properties of the scaffold was performed using histological examination with hematoxy-lin-eosin staining and scanning electron microscopy. The study of the rate of degradation of the scaffold was performed at 37°C and the action of enzymes (trypsin, collagenase). In vivo degradation was studied morphologically after subcutaneous implantation of the studied scaffold to laboratory rats. When morphological study, the scaffold has a high porosity - up to 35-45%. Scaffold shows high thermal stability and does not degrade at 37°C. When exposed to trypsin and type I collagenase solutions, degradation is observed for 540±15 and 200±10 minutes, respectively. When the morphological study of in vivo degradation, scaffold completely degrades within 3 weeks, being replaced by the granulation tissue. The results obtained indicate the possibility of using glutaraldehyde-crosslinked gelatin scaffold modified by dihydroquercetin and arabinogalactan for research in tissue engineering of the lungs.

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