THE CHARACTERISTIC OF MOTION ACTIVITY OF BRONCHIAL MUCOSA CILIATED EPITHELIUM CILIA AT OSMOTIC STRESS IN THE EXPERIMENT IN VITRO

The aim of the research is to study the character and degree of intensiveness of changes in the motion activity of ciliated epithelium (CE) cilia of the bronchial mucosa at immediate effect of hyposmotic stimulus (distilled water) on bronchial biopsy material in the experiment in vitro . 39 patients with bronchial asthma including 14 men and 25 women were examined; all had bronchial fiberoptic scope examination with biopsy of middle lobe bronchus mucosa. For intravital analysis of functional activity of CE cilia the biopsy material was put into the chamber with Hank’s solution at indoor temperature (+25°С). The registration of the motion activity of CE cilia was done with the help of the microsope, the camera with in-built highly sensitive digital full-frame matrix of high definition and a computer. The recording of CE cilia beating was done immediately after the biopsy material was put on the object-plate in the Hank’s solution. Modeling of hypoosmotic stress was carried out by adding 0.01 ml of distilled water into 0.01 ml of Hank’s solution with the help of the dispenser; after that there was an immediate recording of cilia beating and it was repeated in the control moments of the experiment in 1, 2, 3 and 4 minutes. It was found out that the beat frequency of CE cilia fluctuated within the range from 12.2 till 2.7 Hz and was on average 8.73±1.27 Hz. When the distilled water was added into Hank’s solution, there was the decrease of cilia beating frequency till 6.51±1.71 Hz (р<0.001). After the 1^st minute of the experiment the frequency of beating decreased till 5.94±1.57 Hz (by 9%) in comparison with the initial values of the stress reaction. During the 2^nd, 3^rd and 4^th minutes of observation there was a steady tendency of cilia beating frequency decrease (by 13, 15 and 17%, respectively). By the fourth minute of observation the beating frequency was 5.33±1.29 Hz and was statistically different (р=0.035) from the initial value of stress reaction. Thus the results of the research show intensive changes in the motion activity of cilia at the influence of hyposmotic stimulus on bronchial biopsy material in the experiment in vitro . Obtained data allowed to develop the mathematical model which characterizes the behavior of cilia under osmotic stress.

hyposmotic stimulus, osmotic stress, bronchial ciliated epithelium, ciliated epithelium cilia motion activity

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