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Content of 5β-pregnan-3α, 20α-diol in the villous chorion in early pregnancy complicated by cytomegalovirus infection

https://doi.org/10.36604/1998-5029-2020-75-80-85

Abstract

Introduction. Cytomegalovirus infection (CMVI) causes placental abnormalities, almost always accompanied by an imbalance of steroid hormones. It was decided to analyze which stages of hormonal metabolism are most affected by it. One of the markers of placental insufficiency and the development of the threat of termination of pregnancy is 5β-pregnandiol.

Aim. To study the content of 5β-pregnan- 3α, 20α-diol in the placenta of early stages in the exacerbation of CMVI during pregnancy.

Materials and methods. There were studied 98 samples of chorion villi taken during medical abortions at 5-6, 7-8 and 9-10 weeks of pregnancy from practically healthy women (control group) and pregnant women with laboratory-diagnosed CMVI exacerbation (main group). CMVI was diagnosed by determining class M- and G-antibodies using the ELISA, cytomegalovirus DNA was detected by PCR. The content of 5β-pregnan-3α, 20α-diol in the placenta was determined by cytophotometry.

Results. There was a statistically significant (p<0.001) decrease in the content of 5β-pregnan-3α, 20α-diol in samples of villous chorion in pregnancy complicated by CMVI exacerbation at 5-6 weeks (10.08±1,034 and 15.63±1,067 standard units, respectively), 7-8 weeks (12.63±1.098 and 18.56±0.993 standard units, respectively) and 9-10 weeks (17.17±1.107 and 25.90±1.591 standard units, respectively).

Conclusion. The findings suggest that exacerbation of CMVI in the first trimester of pregnancy is associated with impaired production of 5β-pregnane-3α, 20α-diol in the placenta, which may indicate the formation of a threatened abortion.

About the Authors

I. V. Dovzhikova
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation

Inna V. Dovzhikova - PhD, D.Sc. (Biol.), Leading Staff Scientist, Laboratory of Mechanisms of Etiopathogenesis and Recovery Processes of the Respiratory System at Non-Specific Lung Diseases.

22 Kalinina Str., Blagoveshchensk, 675000



I. A. Andrievskaya
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation

Irina A. Andrievskaya - PhD, D.Sc. (Biol.), Professor RAS, Head of Laboratory of Mechanisms of Etiopathogenesis and Recovery Processes of the Respiratory System at Non-Specific Lung Diseases.

22 Kalinina Str., Blagoveshchensk, 675000



N. A. Ishutina
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation

NataM A. Ishutina - PhD, D.Sc. (Biol.), Leading Staff Scientist, Laboratory of Mechanisms of Etiopathogenesis and Recovery Processes of the Respiratory System at Non-Specific Lung Diseases.

22 Kalinina Str., Blagoveshchensk, 675000



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For citations:


Dovzhikova I.V., Andrievskaya I.A., Ishutina N.A. Content of 5β-pregnan-3α, 20α-diol in the villous chorion in early pregnancy complicated by cytomegalovirus infection. Bulletin Physiology and Pathology of Respiration. 2020;(75):80-85. (In Russ.) https://doi.org/10.36604/1998-5029-2020-75-80-85

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ISSN 1998-5029 (Print)