Comparative effectiveness of reamberin and cytoflavin in correction of lipid peroxidation processes induced by ultraviolet radiation

Introduction. The study of the possibility of correct free radical lipid oxidation of membranes in conditions of ultraviolet radiation by the introduction of succinate-containing drugs is of interest, since the search for antioxidant agents is an important area of research in experimental and clinical pharmacology.

Aim. Evaluation of the comparative effectiveness of Reamberin and Cytoflavin (Scientific and technological pharmaceutical company “Polysan”, St.Petersburg) to correct free radical lipid oxidation of rats’ organism membranes induced by ultraviolet radiation.

Materials and methods. The animals were divided into 4 groups and each of them had 20 rats: intact animals (1) which were held in standard conditions of vivarium; the control group (2) in which rats were exposed to ultraviolet radiation during three minutes daily; the experimental group (3) in which before ultraviolet radiation animals had a daily intraabdominal intake of the Reamberin in a dose of 100 mg/kg of succinate (20 mL/kg); the experimental group in which before ultraviolet radiation animals had a daily intraabdominal intake of the Cytoflavin in a dose of 100 mg/kg of succinate (1 mL/kg).

Results. It was found out that in the blood of experimental animals a daily ultraviolet radiation during three minutes contributes to the increase of lipid hydroperoxides level (by 48-53%), of diene conjugate (by 43-48%), and of malonic dialdehyde (by 48-61%) in the setting of the decrease of antioxidant system activity in the blood of intact animals. The introduction of the succinate-containing drugs to rats in the conditions of oxidative stress contributes to the increase of the duration of rats swimming by 11-28% in 7 days of the experiment. The introduction of the succinate containing Reamberin to rats contributes to the decrease in the blood of lipid hydroperoxides by 16-22%, of diene conjugates - by 16-20%, and of malonic dialdehyde by 15-20% in comparison with the rats of the control group. The introduction of the succinate containing Cytoflavin to rats in the conditions of oxidative stress contributes to the decrease in the blood of lipid hydroperoxides by 23-25%, of diene conjugates - by 23-25%, and of malonic dialdehyde by 24-27% in comparison with the rats of the control group. While analyzing the effect of the succinate containing drugs on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the blood of animals was significantly higher by 26-37%, of vitamin E by 25-34%, of catalase by 18-47% in comparison with the same parameters of the rats of the control group.

Conclusion. The application of the succinate containing antioxidants in the conditions of ultraviolet radiation of the organism of animals under experiment leads to the stabilization of the processes of peroxidation against the increase of physical endurance of rats. The intraabdominal introduction in laboratory animals of the Cytoflavin in a dose of 100 mg/kg of succinate prevents the accumulation of lipoperoxidation products and increases the activity of main components of the antioxidant system in rats’ blood plasma, which indirectly exceeds similar effect of the Reamberin in a dose of 100 mg/kg of succinate in the conditions of ultraviolet radiation.

1. Smirnov A.V., Nesterova O.B., Golubev R.V. Succinic acid and its application in medicine. Part II. Application of succinic acid in medicine. Nephrology (Saint-Petersburg). 2014; 18(4):12-24 (in Russian).

2. Eliseeva E.V., Feoktistova Yu.V, Shmykova I.I., Geltser B.I. Analysis of pharmacotherapy in pregnant women. Be-zopasnost' lekarstv i farmakonadzor 2009; (2):23-29 (in Russian).

3. Bondarenko D.A., Smirnov D.V, Simonova N.V, Dorovskikh V.A., Shtarberg M.A. Efficacy of reamberin in the correction of lipid peroxidation processes in the plasma of patients with ovarian cancer. P. A. Herzen Journal of Oncology = Onkologiya. Zhurnal im. PA. Gertsena 2018; 7(6):40-44 (in Russian). doi: https://doi.org/10.17116/onkolog2018706140.

4. Nevzorova V.A., Protopopova M.Yu., Eliseeva E.V., Geltser B.I. NADPH-Diaphorase Activity of Bronchial Epithelium in Chronic Pulmonary Diseases. Morfologiya 1998; 114 (4):77-81 (in Russian).

5. Dorovskikh V.A., Simonova N.V, Korshunova N.V. Adaptogens in the regulation of cold stress. Saabrucken, 2013; 266 (in Russian).

6. Shakhmardanova S. A., Gulevskaya O. N., Khananashvili Y. A., Zelenskaya A. V, Nefedov D. A., Galenko-Yaro-shevsky P. A. Succinic and fumaric acid drugs for prevention and treatment of various diseases. Zhurnal fundamentally meditsiny i biologii 2016; 3:16-30 (in Russian).

7. Zabolotskikh T.V., Simonova N.V, Nosal L.A., Dorovskikh VA., Shtarberg M.A. The experience of using reamberin in the complex treatment of epilepsy in children receiving valproic acid. Rossiyskiy vestnikperinatologii i pediatrii 2019; 64(4):253 (in Russian).

8. Pereverzev D.I., Dorovskikh V A., Simonova N.V, Shtarberg M.A. Effectiveness of Cytoflavin in the correction of lipid peroxidation processes in the blood of patients with acute myocardial infarction. Kardiologiya i serdechno-sosudistaya khirurgiya 2016; 5:42-45 (in Russian). doi: 10.17116/kardio20169542-45

9. Simonova N.V., Dorovskikh V.A., Bondarenko D.A., Nosal' L.A., Shtarberg M.A. Comparative effectiveness of re-maxol and reamberin for carbon tetrachloride-induced hepatic injury treatment in experiment. Eksp. Klin. Farm. 2018; 7:29-33 (in Russian). doi: 10.30906/0869-2092-2018-81-7-29-33

10. Dorovskikh VA., Simonova N.V. Patent 2348079С1 RU. Method and device for experimental modeling of activation of lipid peroxidation processes of biological membranes; published 27.02.2009 (in Russian).

11. Dorovskikh V.A., Simonova N.V, Pereverzev D.I., Yurtaeva E.Yu., Shtarberg M.A. Succinate containing drug for correction of lipid peroxidation processes induced by administration of the carbon tetrachloride. Bulleten’ fiziologii i patologii dyhania = Bulletin Physiology and Pathology of Respiration 2017; (63):75-79 (in Russian). doi: 10.12737/article_58e45635ed6673.27662564

12. Simonova N.V. Phytopreparations in the correction of lipid peroxidation processes of biomembranes induced by ultraviolet radiation. Vestnik Krasnojarskogo gosudarstvennogo agrarnogo universiteta 2009; (2): 119-124 (in Russian).

13. Simonova N.V., Dorovskikh V.A., Kropotov A.V., Anokhina R.A., Shtarberg M.A., Nosal L.A., Maysak A.G., Chernysheva A.A., Kolesov B.V. Effectiveness of succinic acid and reamberin in liver lesions with carbon tetrachloride in the experiment. Amur Medical Journal = Amurskiy meditsinskiy zhurnal 2018; (4):50-53 (in Russian). doi: 10.22448/AMJ.2018.4.50-53

14. Orlov Yu.P. Correction of rheological disorders using solutions of succinates (exchange of experience). St. Petersburg: Tactic Studio; 2016 (in Russian).

15. Simonova N.V., Dorovskikh V.A., Nosal L.A., Kotelnikova M.A., Shtarberg M.A., Maysak A.G., Chernysheva A.A. Efficacy of succinate-containing drug in correction of lipid peroxidation processes induced by introduction of car-bamazepine in experiment. Amur Medical Journal = Amurskiy meditsinskiy zhurnal 2019; (4):45-49 (in Russian). doi: 10.22448/AMJ.2019.4.45-49

16. Liguori I., Russo G., Curcio F., Bulli G., Aran L., Della-Morte D., Gargiulo G, Testa G., Cacciatore F., Bonaduce D., Abete P. Oxidative stress, aging, and diseases. Clin. Interv. Aging 2018; 13:757-772. doi: 10.2147/CIA.S158513