Indicators of vascular-endothelial growth factor and docosahexaenoic acid in pregnant women with threatening course of early pregnancy associated with cytomegalovirus
https://doi.org/10.36604/1998-5029-2020-78-116-121
Abstract
Aim. To determine the indices of vascular endothelial growth factor and docosahexaenoic acid in women with threatened early pregnancy associated with cytomegalovirus infection exacerbation.
Materials and methods. The study included 74 women at a gestation period of 7-12 weeks, of which 38 women with exacerbation of cytomegalovirus infection and the onset of spontaneous miscarriage (main group) and 36 without such pathology (control group). The material for the study was peripheral blood, urine, and scraping of the buccal epithelium. Antibodies IgM and G to cytomegalovirus, low avidity IgG (avidity index) were determined by enzyme-linked immunosorbent assay. Cytomegalovirus DNA was detected by real-time PCR analysis. Indicators of vascular endothelial growth factor were established by enzymelinked immunoassay. The concentration of docosahexaenoic acid was analyzed using capillary gas-liquid chromatography.
Results. The concentration of vascular endothelial growth factor in the blood serum in pregnant women in the main group was 1.7 times (p<0.001) lower than in the control group. A similar dynamics of changes was established for docosahexaenoic acid, the concentration of which was 1.4 times lower than that of the control group (p<0.001).
Conclusion. The data obtained indicate that the exacerbation of cytomegalovirus infection in early pregnancy is associated with a significant decrease in vascular endothelial growth factor and docosahexaenoic acid, which has adverse effects on angiogenesis and growth of placenta, and pregnancy outcome.
Materials and methods. The study included 74 women at a gestation period of 7-12 weeks, of which 38 women with exacerbation of cytomegalovirus infection and the onset of spontaneous miscarriage (main group) and 36 without such pathology (control group). The material for the study was peripheral blood, urine, and scraping of the buccal epithelium. Antibodies IgM and G to cytomegalovirus, low avidity IgG (avidity index) were determined by enzyme-linked immunosorbent assay. Cytomegalovirus DNA was detected by real-time PCR analysis. Indicators of vascular endothelial growth factor were established by enzymelinked immunoassay. The concentration of docosahexaenoic acid was analyzed using capillary gas-liquid chromatography.
Results. The concentration of vascular endothelial growth factor in the blood serum in pregnant women in the main group was 1.7 times (p<0.001) lower than in the control group. A similar dynamics of changes was established for docosahexaenoic acid, the concentration of which was 1.4 times lower than that of the control group (p<0.001).
Conclusion. The data obtained indicate that the exacerbation of cytomegalovirus infection in early pregnancy is associated with a significant decrease in vascular endothelial growth factor and docosahexaenoic acid, which has adverse effects on angiogenesis and growth of placenta, and pregnancy outcome.
About the Authors
N. A. Ishutina
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Nataliа A. Ishutina, PhD, D.Sc. (Biol.), Leading Staff Scientist, Laboratory of Mechanisms of Etiopathogenesis and Recovery Processes of the Respiratory System at Non-Specific Lung Diseases
22 Kalinina Str., Blagoveshchensk, 67500
I. A. Andrievskaya
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Irina A. Andrievskaya, PhD, D.Sc. (Biol.), Professor of RAS, Head of Laboratory of Mechanisms of Etiopathogenesis and Recovery Processes of the Respiratory System at Non-Specific Lung Diseases
22 Kalinina Str., Blagoveshchensk, 67500
N. G. Prikhodko
Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Nikolay G. Prikhodko, Postgraduate student of Laboratory of Mechanisms of Etiopathogenesis and Recovery Processes of the Respiratory System at Non-Specific Lung Diseases
22 Kalinina Str., Blagoveshchensk, 67500
References
1. Lebedeva O.P., Ivanova O.N., Pakhomov S.P., Kalutsky P.V., Suhih N.V. Miscarriages as a result of immune conflict. Problemy reproduktsii 2014; (6):88–91 (in Russian). doi: 10.17116/repro201420688-91
2. Cheshik S.G., Kisteneva L.B. Human cytomegalovirus infection and spontaneous abortion in pregnant women of I and II trimester. Problems of Virology 2016; 61(2):74–78 (in Russian). https://doi.org/10.18821/0507-4088-2016-61-2-74-78
3. Tuleuova Zh.Kh. Miscarriage and infections. Medical Journal of West Kazakhstan 2001; (1):72–73 (in Russian).
4. Petrova K.K. Relative risk assessment of intrauterine infection of the fetus during cytomegalovirus infection in early pregnancy. Acta Biomedica Scientifica (East Siberian Biomedical Journal) 2019; 4(3):45–51 (in Russian). https://doi.org/10.29413/ABS.2019-4.3.6
5. Demchenko N.S., Bashmakova N.V., Tretyakova T.B. Pathogenesis of the pregnancy loss: role of the vascular endothelial growth factor (VEGF-A). Uralskiy meditsinskiy zhurnal=Ural Medical Journal 2012; (11):6 (in Russian).
6. Burton G.J., Charnock-Jones D.S., Jauniaux E. Regulation of vascular growth and function in the human placenta. Reproduction 2009; 138(6):895–902. doi: 10.1530/REP-09-0092
7. González-Pacheco F.R., Deudero J.J., Castellanos M.C., Castilla M.A., Alvarez-Arroyo M.V., Yagüe S., Caramelo C. Mechanisms of endothelial response to oxidative aggression: protective role of autologous VEGF and induction of VEGFR2 by H2O2. Am. J. Physiol. Heart. Circ. Physiol. 2006; 291(3):H1395–401. doi: 10.1152/ajpheart.01277.2005
8. Ul’yanina E.V., Fatkullin I.F. Vascular endothelial growth factor role in predicting vascular disorders in pregnant with fetal growth restriction syndrome. Kazan medical journal 2015; 96(2):220–223 (in Russian). doi: 10.17750/KMJ2015-220
9. Pandya A.D., Das M.K., Sarkar A., Vilasagaram S., Basak S., Duttaroy A.K. Tube formation in the first trimester placental trophoblast cells: Differential effects of angiogenic growth factors and fatty acid. Cell Biol. Int. 2016; 40(6):652–661. doi: 10.1002/cbin.10601
10. Das U.N. Cytokines, angiogenic and antiangiogenic factors and bioactive lipids in preeclampsia. Nutrition 2015; 31(9):1083–1095. doi: 10.1016/j.nut.2015.03.013
11. Hasan A.U., Ohmori K., Konishi K., Igarashi J., Hashimoto T., Kamitori K., Yamaguchi F., Tsukamoto I., Uyama T., Ishihara Y., Noma T., Tokuda M., Kohno M. Eicosapentaenoic acid upregulates VEGF-A through both GPR120 and PPARγ mediated pathways in 3T3-L1 adipocytes. Mol. Cell. Endocrinol. 2015; 406:10–18. doi: 10.1016/j.mce.2015.02.012
12. Carreau J.P., Dubacq J.P. Adaptation of a macro-scale method to the micro-scale for fatty acid methyl transesterification of biological lipid extracts. J. Chromatography 1978; 151(3):384–390. https://doi.org/10.1016/S0021-9673(00)88356-9
13. Andrievskaya I.A., Ishutina N.A., Kutepova O.L. Free-radical oxidation and oxygenation of hemoglobin at exacerbation of cytomegalovirus infection in the second trimester of pregnancy. Acta Biomedica Scientifica (East Siberian Biomedical Journal) 2018; 3(4):9−14 (in Russian). doi: 10/29413/ABS.2018-3.4.1
14. Lebold K.M., Kirkwood J.S., Taylor A.W., Choi J., Barton C.L., Miller G.W., La Du J., Jump D.B., Stevens J.F., Tanguay R.L., Traber M.G. Novel liquid chromatography-mass spectrometry method shows that vitamin E deficiency depletes arachidonic and docosahexaenoic acids in zebrafish (Danio rerio) embryos. Redox Biol. 2013; 2:105–113. doi: 10.1016/j.redox.2013.12.007
15. Rezende F.A., Lapalime E., Qian C.X., Smith L.E., Sangiovanni J.P., Sapieha P. Omega-3 Supplementation Combined With Anti–Vascular Endothelial Growth Factor Lowers Vitreal Levels of Vascular Endothelial Growth Factor in Wet Age-Related Macular Degeneration. Am. J. Ophthalmol. 2014; 158(5):1071–1078. doi: 10.1016/j.ajo.2014.07.036
16. Tan B.K., Adya R., Chen J., Farhatullah S., Heutling D., Mitchell D., Lehnert H., Randeva H.S. Metformin decreases angiogenesis via NF-kappaB and Erk1/2/Erk5 pathways by increasing the antiangiogenic thrombospondin-1. Cardiovasc. Res. 2009; 83(3):566–574. doi: 10.1093/cvr/cvp131
17. Kravtsova E.I., Kutsenko I.I, Kolesnikova N.V, Tomina O.V, Zubko E.A. Clinical and immunological aspects of early reproductive losses in patients with adenomyosis. Kuban Scientific Medical Bulletin 2020; 27(2):81–91 (in Russian). https://doi.org/10.25207/1608-6228-2020-27-2-81-91
18. Tabolova V.K., Korneeva I.E., Donnikov A.E., Burmenskaya O.V., Maslova M.A., Smolnikova V.Yu. The local endometrial expression profile of the growth factor and cytokine genes during the implantation window in chronic endometritis. Obstetrics and Gynecology 2014; (12):74–78 (in Russian).
19. Yakovleva N.Yu., Khazova E.L., Vasil’eva E.Yu., Zazerskaya I.E. Ratio of angiogenic and anti-angiogenic factors in preeclampsia. Arterial’naya Gipertenziya (Arterial Hypertension) 2016; 22(5):488–494 (in Russian). https://doi.org/10.18705/1607-419X-2016-22-5-488-494
Review
For citations:
Ishutina N.A., Andrievskaya I.A., Prikhodko N.G. Indicators of vascular-endothelial growth factor and docosahexaenoic acid in pregnant women with threatening course of early pregnancy associated with cytomegalovirus. Bulletin Physiology and Pathology of Respiration. 2020;(78):116-121. (In Russ.) https://doi.org/10.36604/1998-5029-2020-78-116-121
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