THE STUDY OF ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS OF RS1800470 GENE OF TRANSFORMING GROWTH FACTOR Β1 (TGFB1) AND RS231775 GENE OF CYTOTOXIC T-LYMPHOCYTE-CONNECTED IMMUNOGLOBULIN 4 (CTLA4) WITH THE RISK OF BRONCHIAL ASTHMA DEVELOPMENT

The purpose of the research was to study the influence of single-nucleotide polymorphisms rs1800470 of TGFВ1 gene and rs231775 of CTLA4 gene on the susceptibility to the development of bronchial asthma among the population of Krasnoyarsk. The group of patients with bronchial asthma were 100 people; two control groups, respectively, for each polymorphism - 282 and 338 people. Genome DNA was extracted from 10 ml of venous blood with phenol-chloroform extraction technique. Single nucleotide gene polymorphism was tested with the help of polymerase chain reaction in real time in accordance with the firm producer protocol (probes TaqMan, Applied Biosystems, USA) on the apparatus ABI 7900HT. While studying the polymorphism rs1800470 of gene TGFВ1 for a group of patients with non-allergic asthma the absence of rare homozygous GG was demonstrated. Development of non-allergic asthma significantly associated with genotype AA and AG, in comparison with both the control group (p=0.025) and a group of patients suffering from allergic forms of asthma (p=0.006). If there is evidence of genotypes, non-allergic asthma risk increases compared with the control group (ОR=1.631; 95% CI=1.37-1.94) and with a group of patients with allergic asthma (ОR = 1.128; 95% CI=1.08-3.17). When studying polymorphism rs231775 CTLA4 gene, increased frequency of genotype GG in the group of patients with allergic asthma, including women, was revealed. Taking into consideration the results obtained, it can be concluded that the presence of the genotype GG (ОR=2.036; 95% CI=1.16-3.58) causes an increased risk of allergic asthma in comparison with the control group.

однонуклеотидный полиморфизм, ген rs1800470 TGFb1, ген rs231775 СTLA4, бронхиальная астма, single-nucleotide polymorphism, rs1800470 TGFВ1 gene, rs231775 CTLA4 gene, bronchial asthma

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