GENOTOXIC EFFECT OF OXIDATIVE STRESS IN ELDERLY PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

The aim of the research is the study of genotoxic effects of oxidative stress in elderly patients with stable chronic obstructive pulmonary disease (COPD). The levels of malondialdehyde (MDA), total antioxidant activity (TAA) and 8-hydroxydeoxyguanosine (8-ОНdG) were studied in the blood of somatically healthy persons and middle-aged and elderly COPD patients. The age-dependent 27% increase in oxidative DNA damage marker was found for somatically healthy people (р<0.05). The hyperoxidation and the suppression of antioxidant defense were revealed for mature patients with COPD (67% MDA increase, р<0.001; 25% TАА decrease, р<0.01); it was followed by 126% increase in MDA/TAA ratio (p<0.001). In elderly patients with COPD the levels of MDA were comparable with control group, with 26% decrease in TAA levels (p<0.05). The peroxidation coefficient MDA/TAA was 46% higher than that of the control group (р<0.01), which indicates the shift in balance towards prooxidant processes. The 8-ОНdG levels were 15% higher for elderly patients in comparison with healthy patients of the same age (р<0.05). The intensity of oxidative genome damage also was 97% higher for middle-aged (p<0.001) and 82% for elderly patients (p<0.001) in comparison to healthy people of the same age. Thus, oxidative stress accompanies COPD in middle-aged and elderly patients and causes oxidative DNA damage. The genotoxic effect is most pronounced in elderly patients with COPD, which may be due to the age-dependent suppression of activity of antioxidant ferments, the reduction in DNA repair activity or may indicate the adaptive reorganization of genome. The study of genotoxic effects of oxidative stress in COPD patients considering the age allows to identify molecular pathogenetic mechanisms and develop prevention methods for correction.

окислительный стресс, генотоксический эффект, хроническая обструктивная болезнь легких, старший возраст, oxidative stress, genotoxic effect, chronic obstructive pulmonary disease, elder age

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